PMID- 16858280
OWN - NLM
STAT- MEDLINE
DCOM- 20060911
LR  - 20111117
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 82
IP  - 2
DP  - 2006 Jul 27
TI  - A significant role for histocompatibility in human islet transplantation.
PG  - 180-7
AB  - BACKGROUND: In recent years, transplantation of islets and pancreas has become a 
      viable option for patients debilitated with type I diabetes. The success of islet 
      transplantation has been attributed to the ability to isolate high quality islets 
      for transplantation and capacity to maintain the recipient's immunosuppressive 
      levels within a specific target range following transplantation. The purpose of 
      this study was to determine the role of pretransplant sensitization to human 
      leukocyte antigen (HLA) in islet transplantation. METHODS: We retrospectively 
      analyzed seven patients that were transplanted with islets under the auspices of 
      the Juvenile Diabetes Research Foundation and Islet Cell Resource Center/National 
      Institutes of Health. Humoral sensitization towards donor antigens both prior to 
      and following islet transplantation was detected by FLOW panel reactive 
      antibodies (PRA) and donor-specific cellular sensitization was detected by 
      performing enzyme-linked immunospot assay analysis for cytokines interferon-gamma 
      and interleukin-2. RESULTS: Our analysis demonstrates that humoral and cellular 
      sensitization to histocompatibility antigens prior to and after islet 
      transplantation are associated with the failure of transplanted islets 
      CONCLUSION: Patient selection based on sensitization to donor HLA may be one of 
      the factors crucial for the success of islet transplant. Further, in some 
      patients, rejection of islets can be associated with sensitization to mismatched 
      donor histocompatibility antigens.
FAU - Mohanakumar, T
AU  - Mohanakumar T
AD  - Department of Surgery, Washington University School of Medicine, St. Louis, MO 
      63110, USA. kumart@wustl.edu
FAU - Narayanan, Kishore
AU  - Narayanan K
FAU - Desai, Niraj
AU  - Desai N
FAU - Ramachandran, Sabarinathan
AU  - Ramachandran S
FAU - Shenoy, Surendra
AU  - Shenoy S
FAU - Jendrisak, Martin
AU  - Jendrisak M
FAU - Susskind, Brian M
AU  - Susskind BM
FAU - Olack, Barbara
AU  - Olack B
FAU - Benshoff, Nicholas
AU  - Benshoff N
FAU - Phelan, Donna L
AU  - Phelan DL
FAU - Brennan, Daniel C
AU  - Brennan DC
FAU - Fernandez, Luis A
AU  - Fernandez LA
FAU - Odorico, Jon S
AU  - Odorico JS
FAU - Polonsky, Kenneth S
AU  - Polonsky KS
LA  - eng
GR  - RR16597/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (HLA Antigens)
RN  - 0 (Insulin)
SB  - IM
MH  - Diabetes Mellitus, Type 1/blood/*surgery
MH  - HLA Antigens/immunology
MH  - Histocompatibility/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Insulin/blood
MH  - Islets of Langerhans Transplantation/*immunology
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2006/07/22 09:00
MHDA- 2006/09/12 09:00
CRDT- 2006/07/22 09:00
PHST- 2006/07/22 09:00 [pubmed]
PHST- 2006/09/12 09:00 [medline]
PHST- 2006/07/22 09:00 [entrez]
AID - 00007890-200607270-00009 [pii]
AID - 10.1097/01.tp.0000226161.82581.b2 [doi]
PST - ppublish
SO  - Transplantation. 2006 Jul 27;82(2):180-7. doi: 10.1097/01.tp.0000226161.82581.b2.