PMID- 16858610
OWN - NLM
STAT- MEDLINE
DCOM- 20070202
LR  - 20151123
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 80
IP  - 10
DP  - 2006 Oct
TI  - Expressions of N-methyl-D-aspartate receptors NR2A and NR2B subunit proteins in 
      normal and sulfite-oxidase deficient rat's hippocampus: effect of exogenous 
      sulfite ingestion.
PG  - 671-9
AB  - Sulfites whether ingested or produced through the sulfur-containing amino acids 
      metabolism of the animal are very active molecules and can cause cellular 
      toxicity. Sulfite oxidase (SOX), a heme- and molybdenum containing mitochondrial 
      enzyme, prevents mammalian cells from adverse effects of sulfite toxicity by 
      metabolizing sulfite to sulfate. The present study was aimed to investigate 
      effect of sulfite on the N-methyl-D-aspartate (NMDA) receptor (NMDAR) NR2A and 
      NR2B subunits in hippocampus of normal and SOX-deficient rats. Rats were divided 
      into four groups; (1) control group, which was given rat chow and tap water ad 
      libitum (C), (2) sulfite group, treated with sulfite (25 mg/kg) in drinking water 
      and commercial rat chow ad libitum (S), (3) SOX-deficient group, maintained on 
      high-W/Mo-deficient regimen to produce SOX deficiency (D), and (4) SOX-deficient 
      + sulfite group (DS), prepared as those in the third group and were afterwards 
      given sulfite (25 mg/kg) additionally. Whole treatment schedule were continued 
      for 6 weeks. Sulfite treatment caused a decrease of NR2A and NR2B subunits of the 
      NMDAR in hippocampus of rats in S and DS groups. Interestingly, similar decrement 
      was observed in D group, probably due to increased endogenous sulfite production. 
      In summary, the results indicated that feeding sulfite to the rats may cause 
      down-regulation of NMDARs by degrading NR2A and NR2B subunits of it, which may be 
      considered as a neuro-compensatory mechanism.
FAU - Ozturk, Oktay Hasan
AU  - Ozturk OH
AD  - Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 
      Antalya, Turkey.
FAU - Kucukatay, Vural
AU  - Kucukatay V
FAU - Yonden, Zafer
AU  - Yonden Z
FAU - Agar, Aysel
AU  - Agar A
FAU - Bagci, Huseyin
AU  - Bagci H
FAU - Delibas, Namik
AU  - Delibas N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060621
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Sulfites)
RN  - EC 1.8.3.1 (Sulfite Oxidase)
RN  - VTK01UQK3G (sodium sulfite)
SB  - IM
MH  - Animals
MH  - Cognition/drug effects
MH  - Down-Regulation
MH  - Hippocampus/*drug effects/metabolism
MH  - Liver/drug effects/enzymology
MH  - Male
MH  - Protein Subunits/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*drug effects/metabolism
MH  - Sulfite Oxidase/*deficiency
MH  - Sulfites/*toxicity
MH  - Time Factors
EDAT- 2006/07/22 09:00
MHDA- 2007/02/03 09:00
CRDT- 2006/07/22 09:00
PHST- 2006/05/02 00:00 [received]
PHST- 2006/05/30 00:00 [accepted]
PHST- 2006/07/22 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/07/22 09:00 [entrez]
AID - 10.1007/s00204-006-0125-x [doi]
PST - ppublish
SO  - Arch Toxicol. 2006 Oct;80(10):671-9. doi: 10.1007/s00204-006-0125-x. Epub 2006 
      Jun 21.