PMID- 16861641
OWN - NLM
STAT- MEDLINE
DCOM- 20060829
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 74
IP  - 8
DP  - 2006 Aug
TI  - Effect of lung surfactant collectins on bronchoalveolar macrophage interaction 
      with Blastomyces dermatitidis: inhibition of tumor necrosis factor alpha 
      production by surfactant protein D.
PG  - 4549-56
AB  - Alveolar surfactant modulates the antimicrobial function of bronchoalveolar 
      macrophages (BAM). Little is known about the effect of surfactant-associated 
      proteins in bronchoalveolar lavage fluid (BALF) on the interaction of BAM and 
      Blastomyces dermatitidis. We investigated BALF enhancement or inhibition of 
      TNF-alpha production by BAM stimulated by B. dermatitidis. BAM from CD-1 mice 
      were stimulated with B. dermatitidis without or with normal BALF, surfactant 
      protein A-deficient (SP-A-/-) or surfactant protein D-deficient (SP-D-/-) BALF, 
      or a mixture of SP-A-/- and SP-D-/- BALF. An enzyme-linked immunosorbent assay 
      was used to measure tumor necrosis factor alpha (TNF-alpha) in culture 
      supernatants. BALFs were standardized in protein concentration. BAM plus B. 
      dermatitidis (BAM-B. dermatitidis) TNF-alpha production was inhibited > or = 47% 
      by BALF or SP-A-/- BALF (at 290 or 580 microg of protein/ml, P < 0.05 to 0.01); 
      in contrast, SP-D-/- BALF did not significantly inhibit TNF-alpha production. If 
      SP-A-/- BALF was mixed in equal amounts with SP-D-/- BALF, TNF-alpha production 
      by BAM-B. dermatitidis was inhibited (P < 0.01). Finally, pure SP-D added to 
      SP-D-/- BALF inhibited TNF-alpha production by BAM-B. dermatitidis (P < 0.01). B. 
      dermatitidis incubated with BALF and washed, plus BAM, stimulated 63% less 
      production of TNF-alpha than did unwashed B. dermatitidis (P < 0.05). SP-D was 
      detected by anti-SP-D antibody on BALF-treated unwashed B. dermatitidis in an 
      immunofluorescence assay (IFA). The BALF depleted by a coating of B. dermatitidis 
      lost the ability to inhibit TNF-alpha production (P < 0.05). 1,3-beta-Glucan was 
      a good stimulator of BAM for TNF-alpha production and was detected on B. 
      dermatitidis by IFA. beta-Glucan incubated with BALF inhibited the binding of 
      SP-D in BALF to B. dermatitidis as demonstrated by IFA. Our data suggest that 
      SP-D in BALF binds beta-glucan on B. dermatitidis, blocking BAM access to 
      beta-glucan, thereby inhibiting TNF-alpha production. Thus, whereas BALF 
      constituents commonly mediate antimicrobial activity, B. dermatitidis may utilize 
      BALF constituents, such as SP-D, to blunt the host defensive reaction; this 
      effect could reduce inflammation and tissue destruction but could also promote 
      disease.
FAU - Lekkala, Madhavi
AU  - Lekkala M
AD  - Division of Infectious Diseases, Department of Medicine, Santa Clara Valley 
      Medical Center, 751 South Bascom Ave., San Jose, California 95128-2699, USA.
FAU - LeVine, Ann Marie
AU  - LeVine AM
FAU - Linke, Michael J
AU  - Linke MJ
FAU - Crouch, Erika C
AU  - Crouch EC
FAU - Linders, Bruce
AU  - Linders B
FAU - Brummer, Elmer
AU  - Brummer E
FAU - Stevens, David A
AU  - Stevens DA
LA  - eng
GR  - P01 HL029594/HL/NHLBI NIH HHS/United States
GR  - R01 HL044015/HL/NHLBI NIH HHS/United States
GR  - HL-29594/HL/NHLBI NIH HHS/United States
GR  - HL-44015/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Pulmonary Surfactant-Associated Protein D)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (beta-Glucans)
SB  - IM
MH  - Animals
MH  - Blastomyces/immunology/*pathogenicity
MH  - Blastomycosis/immunology/microbiology
MH  - Bronchoalveolar Lavage Fluid/immunology/microbiology
MH  - Macrophage Activation
MH  - Macrophages, Alveolar/drug effects/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Pulmonary Surfactant-Associated Protein D/deficiency/metabolism/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/*drug effects
MH  - beta-Glucans/metabolism
PMC - PMC1539632
EDAT- 2006/07/25 09:00
MHDA- 2006/08/30 09:00
PMCR- 2006/12/01
CRDT- 2006/07/25 09:00
PHST- 2006/07/25 09:00 [pubmed]
PHST- 2006/08/30 09:00 [medline]
PHST- 2006/07/25 09:00 [entrez]
PHST- 2006/12/01 00:00 [pmc-release]
AID - 74/8/4549 [pii]
AID - 0243-06 [pii]
AID - 10.1128/IAI.00243-06 [doi]
PST - ppublish
SO  - Infect Immun. 2006 Aug;74(8):4549-56. doi: 10.1128/IAI.00243-06.