PMID- 16861756
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20131121
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 334
DP  - 2006
TI  - PRINS for the detection of gene deletions in cancer.
PG  - 105-13
AB  - The chromosomal regions 13q14 and 17p13 often are found rearranged in 
      hematopoietic tumors in humans, but the rearrangements can be subtle and can 
      escape detection on gross cytogenetic analysis. For example, submicroscopic 
      perturbations of the RB1 and p53 tumor suppressor genes, located in 13q14 and 
      17p13, respectively, frequently occur in leukemias; this has been confirmed by 
      molecular methods such as fluorescence in situ hybridization (FISH). Our group 
      modified the primed in situ labeling (PRINS) method to study RB1 and p53 in 
      cultured bone marrow cells from leukemia patients with known deletions within the 
      13q14 or 17p13 regions. Locus-specific oligonucleotide probes ("PRINS primers") 
      were annealed to chromosomal DNA on glass slides and extended in the presence of 
      the four trinucleotide precursors, biotin-16-dUTP, and Taq DNA polymerase. After 
      addition of avidin-conjugated fluorophores, the resulting signals could be 
      visualized by fluorescence microscopy in metaphase spreads and interphase nuclei 
      of controls but were absent in the corresponding preparations from patients. The 
      results of these and similar studies suggest that with further development, PRINS 
      might be used as a convenient and rapid alternative to FISH in the delineation of 
      deletions involving single genes or unique sequences.
FAU - Tharapel, Avirachan T
AU  - Tharapel AT
AD  - Department of Pediatrics, University of Tennessee, Memphis, TN, USA.
FAU - Wachtel, Stephen S
AU  - Wachtel SS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Neoplasm)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Biotin/metabolism
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - DNA, Neoplasm/*analysis/genetics
MH  - *Gene Deletion
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - Neoplasms/*genetics
MH  - Primed In Situ Labeling/*methods
EDAT- 2006/07/25 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/07/25 09:00
PHST- 2006/07/25 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/07/25 09:00 [entrez]
AID - 1-59745-068-5:105 [pii]
AID - 10.1385/1-59745-068-5:105 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;334:105-13. doi: 10.1385/1-59745-068-5:105.