PMID- 16861760
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20061115
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 334
DP  - 2006
TI  - Dual-color PRINS for in situ detection of fetal cells in maternal blood.
PG  - 141-9
AB  - Fetal nucleated cells circulating in the peripheral blood during pregnancy are 
      potential targets for noninvasive genetic testing. Fluorescence in situ 
      hybridization (FISH) frequently is used to quantify the total number of fetal 
      cells in peripheral blood of pregnant women. We describe an alternative molecular 
      cytogenetic procedure that is the primed in situ labeling (PRINS). This technique 
      consists of annealing oligonucleotides specific to individual chromosome targets 
      and in situ elongation using Taq DNA polymerase to incorporate labeled dUTPs. The 
      sites of the newly synthesized DNA sequences were revealed as fluorescent signals 
      using an immunochemical reaction. The dual-color PRINS was specifically performed 
      for simultaneous detection of two chromosome targets, X and Y. The fluorescent 
      signals corresponding to chromosomes X and Y were displayed as red and green 
      color spots, respectively. The sensitivity and specificity of PRINS are similar 
      to FISH and allow us to efficiently and reliably detect fetal cells in maternal 
      blood. Moreover, dual-color PRINS is faster and more cost-effective than FISH.
FAU - Krabchi, Kada
AU  - Krabchi K
AD  - Service of Genetics, Department of Pediatrics, Faculty of Medicine and Health 
      Sciences, University of Sherbrooke, Sherbrooke, Quebec, Canada.
FAU - Gadji, Macoura
AU  - Gadji M
FAU - Yan, Ju
AU  - Yan J
FAU - Drouin, Regen
AU  - Drouin R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - *Color
MH  - Female
MH  - Fetus/*cytology
MH  - Humans
MH  - Pregnancy/*blood
MH  - Primed In Situ Labeling/*methods
EDAT- 2006/07/25 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/07/25 09:00
PHST- 2006/07/25 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/07/25 09:00 [entrez]
AID - 1-59745-068-5:141 [pii]
AID - 10.1385/1-59745-068-5:141 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;334:141-9. doi: 10.1385/1-59745-068-5:141.