PMID- 16864656
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20211203
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 174
IP  - 3
DP  - 2006 Jul 31
TI  - Transport of PIP3 by GAKIN, a kinesin-3 family protein, regulates neuronal cell 
      polarity.
PG  - 425-36
AB  - Phosphatidylinositol-(3,4,5)-trisphosphate (PIP3), a product of 
      phosphatidylinositol 3-kinase, is an important second messenger implicated in 
      signal transduction and membrane transport. In hippocampal neurons, the 
      accumulation of PIP3 at the tip of neurite initiates the axon specification and 
      neuronal polarity formation. We show that guanylate kinase-associated kinesin 
      (GAKIN), a kinesin-like motor protein, directly interacts with a PIP3-interacting 
      protein, PIP3BP, and mediates the transport of PIP3-containing vesicles. 
      Recombinant GAKIN and PIP3BP form a complex on synthetic liposomes containing 
      PIP3 and support the motility of the liposomes along microtubules in vitro. In 
      PC12 cells and cultured hippocampal neurons, transport activity of GAKIN 
      contributes to the accumulation of PIP3 at the tip of neurites. In hippocampal 
      neurons, altered accumulation of PIP3 by overexpression of GAKIN constructs led 
      to the loss of the axonally differentiated neurites. Together, these results 
      suggest that, in neurons, the GAKIN-PIP3BP complex transports PIP3 to the neurite 
      ends and regulates neuronal polarity formation.
FAU - Horiguchi, Kaori
AU  - Horiguchi K
AD  - Department of Pharmacology and Cancer Center, University of Illinois College of 
      Medicine, Chicago, IL 60612, USA.
FAU - Hanada, Toshihiko
AU  - Hanada T
FAU - Fukui, Yasuhisa
AU  - Fukui Y
FAU - Chishti, Athar H
AU  - Chishti AH
LA  - eng
GR  - R01 CA094414/CA/NCI NIH HHS/United States
GR  - CA 94414/CA/NCI NIH HHS/United States
GR  - HL60755/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060724
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Liposomes)
RN  - 0 (Molecular Motor Proteins)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (phosphatidylinositol 3,4,5-triphosphate)
RN  - EC 3.6.1.- (KIF13B protein, human)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Animals
MH  - Biological Transport
MH  - COS Cells
MH  - Cattle
MH  - *Cell Polarity
MH  - Cells, Cultured
MH  - Chlorocebus aethiops
MH  - Humans
MH  - Kinesins/chemistry/*metabolism
MH  - Liposomes/metabolism
MH  - Mice
MH  - Microtubules/metabolism
MH  - Models, Biological
MH  - Molecular Motor Proteins/metabolism
MH  - Neurons/*cytology
MH  - PC12 Cells
MH  - Phosphatidylinositol Phosphates/*metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Rats
PMC - PMC2064238
EDAT- 2006/07/26 09:00
MHDA- 2006/09/20 09:00
PMCR- 2007/01/31
CRDT- 2006/07/26 09:00
PHST- 2006/07/26 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2006/07/26 09:00 [entrez]
PHST- 2007/01/31 00:00 [pmc-release]
AID - jcb.200604031 [pii]
AID - 200604031 [pii]
AID - 10.1083/jcb.200604031 [doi]
PST - ppublish
SO  - J Cell Biol. 2006 Jul 31;174(3):425-36. doi: 10.1083/jcb.200604031. Epub 2006 Jul 
      24.