PMID- 16865900
OWN - NLM
STAT- MEDLINE
DCOM- 20060821
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 69
IP  - 7
DP  - 2006 Jul
TI  - Identification of Salmonella enterica serovar Typhimurium using specific PCR 
      primers obtained by comparative genomics in Salmonella serovars.
PG  - 1653-61
AB  - Salmonella enterica serovar Typhimurium is a major foodborne pathogen throughout 
      the world. Until now, the specific target genes for the detection and 
      identification of serovar Typhimurium have not been developed. To determine the 
      specific probes for serovar Typhimurium, the genes of serovar Typhimurium LT2 
      that were expected to be unique were selected with the BLAST (Basic Local 
      Alignment Search Tool) program within GenBank. The selected genes were compared 
      with 11 genomic sequences of various Salmonella serovars by BLAST. Of these 
      selected genes, 10 were expected to be specific to serovar Typhimurium and were 
      not related to virulence factor genes of Salmonella pathogenicity island or to 
      genes of the O and H antigens of Salmonella. Primers for the 10 selected genes 
      were constructed, and PCRs were evaluated with various genomic DNAs of Salmonella 
      and non-Salmonella strains for the specific identification of Salmonella serovar 
      Typhimurium. Among all the primer sets for the 10 genes, STM4497 showed the 
      highest degree of specificity to serovar Typhimurium. In this study, a specific 
      primer set for Salmonella serovar Typhimurium was developed on the basis of the 
      comparison of genomic sequences between Salmonella serovars and was validated 
      with PCR. This method of comparative genomics to select target genes or sequences 
      can be applied to the specific detection of microorganisms.
FAU - Kim, H J
AU  - Kim HJ
AD  - Institute of Life Sciences and Resources and Graduate School of Biotechnology, 
      Kyung Hee University, Suwon, 449-701, Korea.
FAU - Park, S H
AU  - Park SH
FAU - Lee, T H
AU  - Lee TH
FAU - Nahm, B H
AU  - Nahm BH
FAU - Chung, Y H
AU  - Chung YH
FAU - Seo, K H
AU  - Seo KH
FAU - Kim, H Y
AU  - Kim HY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA, Bacterial/*analysis
MH  - Food Contamination/*analysis
MH  - *Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - Salmonella Food Poisoning/diagnosis/microbiology
MH  - Salmonella enterica/*classification/*isolation & purification
MH  - Salmonella typhimurium/classification/isolation & purification
MH  - Sensitivity and Specificity
MH  - Sequence Alignment
MH  - Species Specificity
MH  - Virulence Factors/genetics
EDAT- 2006/07/27 09:00
MHDA- 2006/08/22 09:00
CRDT- 2006/07/27 09:00
PHST- 2006/07/27 09:00 [pubmed]
PHST- 2006/08/22 09:00 [medline]
PHST- 2006/07/27 09:00 [entrez]
AID - S0362-028X(22)07503-2 [pii]
AID - 10.4315/0362-028x-69.7.1653 [doi]
PST - ppublish
SO  - J Food Prot. 2006 Jul;69(7):1653-61. doi: 10.4315/0362-028x-69.7.1653.