PMID- 16866625
OWN - NLM
STAT- MEDLINE
DCOM- 20060914
LR  - 20181113
IS  - 0897-7151 (Print)
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 23
IP  - 7
DP  - 2006 Jul
TI  - Functional reinnervation of the canine bladder after spinal root transection and 
      immediate end-on-end repair.
PG  - 1125-36
AB  - The goal of this study was to transect and immediately repair ventral roots, 
      selected by their ability to stimulate bladder contraction, to assess the 
      feasibility of bladder reinnervation in a canine model. Brain-derived 
      neurotrophic factor (BDNF) was delivered via an osmotic pump (0.5 or 5 mg/mL) to 
      a cuff surrounding the reanastomosis site to the two root bundles on one side. 
      Electrodes were implanted bilaterally immediately proximal to the site of 
      surgical reanastomosis. Results were compared to four root-intact, control 
      animals that also received bilateral electrode implantation. At 6-12 months 
      post-surgery, five of eight nerve transected and repaired animals showed 
      increased pressure and bladder emptying during electrical stimulation of the 
      repaired ventral roots contralateral to the BDNF delivery side. Nerve tracing 
      studies one year postoperatively determined the repaired roots to be S1 and S2 
      and showed regrowth of axons from the spinal cord to nerve sites proximal to the 
      repair site and to the bladder, and the presence of neurofilament-labeled axons 
      growing across the ventral root repair site. In conclusion, transected ventral 
      and dorsal roots in the sacral spine can be repaired and are capable of 
      functionally reinnervating the urinary bladder. This feasibility study paves the 
      way for future studies utilizing other more proximal motor nerves to bypass the 
      transection site for bladder reinnervation.
FAU - Ruggieri, Michael R
AU  - Ruggieri MR
AD  - Department of Urology, Temple University School of Medicine, Philadelphia, 
      Pennsylvania 19140, USA. rugg@temple.edu
FAU - Braverman, Alan S
AU  - Braverman AS
FAU - D'Andrea, Linda
AU  - D'Andrea L
FAU - Simpkiss, Bernadette
AU  - Simpkiss B
FAU - Kozin, Scott H
AU  - Kozin SH
FAU - Pontari, Michel A
AU  - Pontari MA
FAU - Betz, Randall
AU  - Betz R
FAU - Barbe, Mary F
AU  - Barbe MF
LA  - eng
GR  - R01 DK043333/DK/NIDDK NIH HHS/United States
GR  - R01 NS070267/NS/NINDS NIH HHS/United States
GR  - R01 NS070267-01/NS/NINDS NIH HHS/United States
GR  - R01-DK43333/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Dogs
MH  - Female
MH  - *Nerve Regeneration/drug effects/physiology
MH  - Spinal Nerve Roots/drug effects/*injuries/*surgery
MH  - Urinary Bladder/drug effects/*innervation/*surgery
PMC - PMC3285498
MID - NIHMS353510
EDAT- 2006/07/27 09:00
MHDA- 2006/09/15 09:00
PMCR- 2012/02/23
CRDT- 2006/07/27 09:00
PHST- 2006/07/27 09:00 [pubmed]
PHST- 2006/09/15 09:00 [medline]
PHST- 2006/07/27 09:00 [entrez]
PHST- 2012/02/23 00:00 [pmc-release]
AID - 10.1089/neu.2006.23.1125 [doi]
PST - ppublish
SO  - J Neurotrauma. 2006 Jul;23(7):1125-36. doi: 10.1089/neu.2006.23.1125.