PMID- 16867981
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 281
IP  - 39
DP  - 2006 Sep 29
TI  - PDZK1 is required for maintaining hepatic scavenger receptor, class B, type I 
      (SR-BI) steady state levels but not its surface localization or function.
PG  - 28975-80
AB  - PDZK1 is a multi-PDZ domain-containing adaptor protein that binds to the C 
      terminus of the high density lipoprotein receptor, scavenger receptor, class B, 
      type I (SR-BI), and controls the posttranscriptional, tissue-specific expression 
      of this lipoprotein receptor. In the absence of PDZK1 (PDZK1(-/-) mice), murine 
      hepatic SR-BI protein levels are very low (<5% of control). As a consequence, 
      abnormal plasma lipoprotein metabolism ( approximately 1.5-1.7-fold increased 
      total plasma cholesterol carried in both normal size and abnormally large high 
      density lipoprotein particles) resembles, but is not as severely defective as, 
      that in SR-BI(-/-) mice. Here we show that the total plasma cholesterol levels 
      and size distribution of lipoproteins are virtually identical in SR-BI(-/-) and 
      SR-BI(-/-)/PDZK1(-/-) mice, indicating that most, if not all of the effects of 
      PDZK1 on lipoprotein metabolism are likely because of the effects of PDZK1 on 
      SR-BI. Hepatic overexpression of wild-type SR-BI in PDZK1(-/-) mice restored near 
      or greater than normal levels of cell surface-expressed, functional SR-BI protein 
      levels in the livers of SR-BI(-/-)/PDZK1(-/-) mice and consequently restored 
      apparently normal lipoprotein metabolism in the absence of PDZK1. Thus, PDZK1 is 
      important for maintaining adequate steady state levels of SR-BI in the liver but 
      is not essential for cell surface expression or function of hepatic SR-BI.
FAU - Yesilaltay, Ayce
AU  - Yesilaltay A
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge, 
      Massachusetts 02139, USA.
FAU - Kocher, Olivier
AU  - Kocher O
FAU - Pal, Rinku
AU  - Pal R
FAU - Leiva, Andrea
AU  - Leiva A
FAU - Quinones, Veronica
AU  - Quinones V
FAU - Rigotti, Attilio
AU  - Rigotti A
FAU - Krieger, Monty
AU  - Krieger M
LA  - eng
GR  - HL64737/HL/NHLBI NIH HHS/United States
GR  - HL66105/HL/NHLBI NIH HHS/United States
GR  - TW006153/TW/FIC NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060725
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CD36 Antigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PDZK1 protein, mouse)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - CD36 Antigens/metabolism/*physiology
MH  - Carrier Proteins/metabolism/*physiology
MH  - Cell Membrane/metabolism
MH  - Cholesterol/blood
MH  - Gene Expression Regulation
MH  - Genotype
MH  - Intracellular Signaling Peptides and Proteins/chemistry/*physiology
MH  - Lipoproteins/metabolism
MH  - Membrane Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Transgenes
EDAT- 2006/07/27 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/07/27 09:00
PHST- 2006/07/27 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/07/27 09:00 [entrez]
AID - S0021-9258(19)33990-0 [pii]
AID - 10.1074/jbc.M603802200 [doi]
PST - ppublish
SO  - J Biol Chem. 2006 Sep 29;281(39):28975-80. doi: 10.1074/jbc.M603802200. Epub 2006 
      Jul 25.