PMID- 16870944
OWN - NLM
STAT- MEDLINE
DCOM- 20070226
LR  - 20220129
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 75
IP  - 5
DP  - 2006 Nov
TI  - Vasopressin regulation of the proestrous luteinizing hormone surge in wild-type 
      and Clock mutant mice.
PG  - 778-84
AB  - In the female mouse, ovulation and estrous cyclicity are under both hormonal and 
      circadian control. We have shown that mice with a mutation in the core circadian 
      gene Clock have abnormal estrous cycles and do not have a luteinizing hormone 
      (LH) surge on the afternoon of proestrus due to a defect at the hypothalamic 
      level. In the present study, we tested the hypotheses that vasopressin (AVP) can 
      act as a circadian signal to regulate the proestrous release of LH, and that this 
      signal is deficient in the Clock mutant. We found that Avp expression in the 
      suprachiasmatic nucleus (SCN) and AVP 1a receptor (Avpr1a) expression in the 
      hypothalamus is reduced in Clock mutant mice compared to wild-type mice. 
      Intracerebroventricular (i.c.v.) injection of AVP on the afternoon of proestrus 
      is sufficient to induce LH secretion, which reaches surge levels in 50% of Clock 
      mutant mice. The effect of AVP on the Clock mutant LH surge is mediated by 
      AVPR1A, as co-infusion of AVP and an AVPR1A-specific antagonist prevents AVP 
      induction of LH release, although infusion of an AVPR1A antagonist into wild-type 
      mice failed to prevent a proestrous LH surge. These results suggest that reduced 
      hypothalamic AVP signaling plays a role in the absence of the proestrous LH surge 
      in Clock mutant mice. The results also support the hypothesis that AVP produced 
      by the SCN may be a circadian signal that regulates LH release.
FAU - Miller, Brooke H
AU  - Miller BH
AD  - Department of Neurobiology and Physiology, Northwestern University, Evanston, 
      Illinois 60201, USA. bmiller@scripps.edu
FAU - Olson, Susan Losee
AU  - Olson SL
FAU - Levine, Jon E
AU  - Levine JE
FAU - Turek, Fred W
AU  - Turek FW
FAU - Horton, Teresa H
AU  - Horton TH
FAU - Takahashi, Joseph S
AU  - Takahashi JS
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01 HD020677/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060726
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (AVPR1a receptor, mouse)
RN  - 0 (Antidiuretic Hormone Receptor Antagonists)
RN  - 0 (Receptors, Vasopressin)
RN  - 0 (Trans-Activators)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 2.3.1.48 (CLOCK Proteins)
RN  - EC 2.3.1.48 (Clock protein, mouse)
SB  - IM
MH  - Animals
MH  - Antidiuretic Hormone Receptor Antagonists
MH  - Arginine Vasopressin/antagonists & inhibitors/*metabolism
MH  - CLOCK Proteins
MH  - Female
MH  - Gene Expression
MH  - Genotype
MH  - Luteinizing Hormone/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proestrus/*metabolism
MH  - Receptors, Vasopressin/metabolism
MH  - Suprachiasmatic Nucleus/*metabolism
MH  - Time Factors
MH  - Trans-Activators/genetics/*metabolism
EDAT- 2006/07/28 09:00
MHDA- 2007/02/27 09:00
CRDT- 2006/07/28 09:00
PHST- 2006/07/28 09:00 [pubmed]
PHST- 2007/02/27 09:00 [medline]
PHST- 2006/07/28 09:00 [entrez]
AID - biolreprod.106.052845 [pii]
AID - 10.1095/biolreprod.106.052845 [doi]
PST - ppublish
SO  - Biol Reprod. 2006 Nov;75(5):778-84. doi: 10.1095/biolreprod.106.052845. Epub 2006 
      Jul 26.