PMID- 16872569
OWN - NLM
STAT- MEDLINE
DCOM- 20070808
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 30
IP  - 6
DP  - 2006 Jul-Aug
TI  - Validation of the immunalysis microplate ELISA for the detection of 
      methamphetamine in hair.
PG  - 380-5
AB  - The object of this study was to validate the Immunalysis Methamphetamine 
      Microplate ELISA for detecting methamphetamine in hair. Twenty-nine scalp hair 
      samples were obtained as routine cases submitted to the National Institute of 
      Scientific Investigation in Seoul by the police. The hair samples were washed 
      with 0.1% sodium dodecyl sulfate, distilled water, and dichloromethane. The 
      samples were screened using the Immunalysis Methamphetamine Microplate ELISA and 
      confirmed using gas chromatography-mass spectrometry (GC-MS). Twenty-eight hair 
      samples were screened and confirmed as positive for methamphetamine. For ELISA 
      analysis, the samples were extracted by incubation in monobasic phosphate buffer 
      for 1 h at 60 degrees C. For GC-MS, the samples were extracted for 20 h in 
      methanol containing 1% hydrochloric acid. The methanol/acid solution was 
      evaporated to dryness and the resulting residue was derivatized with 
      trifluoroacetic anhydride. Methamphetamine and amphetamine were detected using 
      selective ion monitoring (SIM) mode. The Immunalysis Methamphetamine Microplate 
      ELISA demonstrated a sensitivity and specificity of 97% and 100%, respectively, 
      using a cut-off concentration of 0.5 ng/mg d-methamphetamine. The ELISA kit 
      showed 63% cross-reactivity with d,l-methamphetamine and did not cross-react to 
      any significant extent with the licit l-methamphetamine isomer. The intra- and 
      interassay precisions were 2.5% and 3.7%, respectively.
FAU - Han, Eunyoung
AU  - Han E
AD  - Department of Narcotics Analysis, National Institute of Scientific Investigation, 
      331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
FAU - Miller, Eleanor
AU  - Miller E
FAU - Lee, Juseon
AU  - Lee J
FAU - Park, Yonghoon
AU  - Park Y
FAU - Lim, Miae
AU  - Lim M
FAU - Chung, Heesun
AU  - Chung H
FAU - Wylie, Fiona M
AU  - Wylie FM
FAU - Oliver, John S
AU  - Oliver JS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Central Nervous System Stimulants)
RN  - 44RAL3456C (Methamphetamine)
SB  - IM
MH  - Central Nervous System Stimulants/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Evaluation Studies as Topic
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hair/*chemistry
MH  - Humans
MH  - Methamphetamine/*analysis
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/*methods
EDAT- 2006/07/29 09:00
MHDA- 2007/08/09 09:00
CRDT- 2006/07/29 09:00
PHST- 2006/07/29 09:00 [pubmed]
PHST- 2007/08/09 09:00 [medline]
PHST- 2006/07/29 09:00 [entrez]
AID - 10.1093/jat/30.6.380 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2006 Jul-Aug;30(6):380-5. doi: 10.1093/jat/30.6.380.