PMID- 16873559
OWN - NLM
STAT- MEDLINE
DCOM- 20061226
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 291
IP  - 6
DP  - 2006 Dec
TI  - bFGF induces an earlier expression of nephrogenic proteins after ischemic acute 
      renal failure.
PG  - R1677-87
AB  - Recovery from acute renal failure (ARF) requires the replacement of injured cells 
      with new cells that restore tubule epithelial integrity. We described recently 
      the expression of a wide range of nephrogenic proteins in tubular cells after ARF 
      induced by ischemia-reperfusion (I/R) (Villanueva S, Cespedes C, and Vio CP. Am J 
      Physiol Regul Integr Comp Physiol 290: R861-R870, 2006). These markers, namely, 
      Vimentin, neural cell adhesion molecules (Ncam), basic fibroblast growth factor 
      (bFGF), paired homeobox-2 (Pax-2), bone morphogene protein-7 (BMP-7), Noggin, 
      Lim-1, Engrailed, Smad, phospho-Smad, hypoxia-induced factor-1alpha (HIF-1alpha), 
      VEGF, and Tie-2, are expressed in a time frame similar to that observed in normal 
      kidney development. bFGF participates in early kidney development as a morphogen 
      involved in mesenchyme/epithelial transition, and it is reexpressed in the 
      recovery phase of ARF. To test the hypothesis that bFGF can accelerate the 
      regeneration after renal damage, we used recombinant bFGF and studied the 
      expression pattern of the above described morphogens in ARF. Male Sprague-Dawley 
      rats were subjected to 30 min of renal ischemic injury and were injected with 
      bFGF 30 microg/kg followed by reperfusion. Rats were killed and the expression of 
      nephrogenic proteins were analyzed by immunohistochemistry and Western blot 
      analysis. In the animals subjected to I/R treated with bFGF, we observed a 12- to 
      24-h earlier and more abundant reexpression of the proteins Ncam, bFGF, Pax-2, 
      BMP-7, Noggin, Lim-1, Engrailed, VEGF, and Tie-2 than the I/R untreated rats. In 
      addition, we observed a reduction in renal damage markers ED-1 and alpha-smooth 
      muscle actin. These results indicate that bFGF can participate in the 
      regeneration process and suggest that the treatment with bFGF can induce an 
      earlier regeneration process after ischemic acute renal failure.
FAU - Villanueva, Sandra
AU  - Villanueva S
AD  - Dept. de Fisiologia, Pontificia Universidad Catolica de Chile, Casilla 114-D, 
      Santiago, Chile. svillanu@bio.puc.cl
FAU - Cespedes, Carlos
AU  - Cespedes C
FAU - Gonzalez, Alexis
AU  - Gonzalez A
FAU - Vio, Carlos P
AU  - Vio CP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060727
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Acute Kidney Injury/etiology/*metabolism/pathology
MH  - Animals
MH  - Bone Morphogenetic Proteins/*metabolism
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Gene Expression/drug effects
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regeneration/drug effects/*physiology
MH  - Reperfusion Injury/complications/*metabolism/pathology
EDAT- 2006/07/29 09:00
MHDA- 2006/12/27 09:00
CRDT- 2006/07/29 09:00
PHST- 2006/07/29 09:00 [pubmed]
PHST- 2006/12/27 09:00 [medline]
PHST- 2006/07/29 09:00 [entrez]
AID - 00023.2006 [pii]
AID - 10.1152/ajpregu.00023.2006 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2006 Dec;291(6):R1677-87. doi: 
      10.1152/ajpregu.00023.2006. Epub 2006 Jul 27.