PMID- 16874382
OWN - NLM
STAT- MEDLINE
DCOM- 20070509
LR  - 20060728
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 6
IP  - 8
DP  - 2006 Aug
TI  - Low melting point agarose as a protection layer in photolithographic patterning 
      of aligned binary proteins.
PG  - 1080-5
AB  - A novel photolithography method to build aligned patterns of two different 
      proteins is presented. Chessboard patterns of 125 microm x 125 microm squares are 
      constructed on a silicon dioxide substrate, using standard photoresist 
      chemistries in combination with low-temperature oxygen plasma etching. 
      Low-melting-point agarose (LMPA) is used to protect underlying protein layers 
      and, at the appropriate stage, the digestive enzyme GELase (EPICENTRE) is used to 
      selectively remove the prophylactic LMPA layers. Two antibodies, mouse-IgG and 
      human-IgG, were immobilized and patterned by this procedure. The patterned 
      antibodies maintained the specificity of their antigen-antibody binding, as 
      demonstrated by fluorescence microscopy. In addition, normalized fluorescence 
      intensity profiles illustrate that the patterned proteins layers are uniform 
      (standard deviations below 0.05). Finally, a trypsin activity test was conducted 
      to probe the effect of the patterning protocol on immobilized enzymes; the 
      results imply that this photolithographic process using LMPA as a protection 
      layer preserves 70% of immobilized enzyme activity.
FAU - Lee, Lap Man
AU  - Lee LM
AD  - Dept of Aerospace & Mechanical Engineering, The University of Arizona, Tucson, AZ 
      85721, USA.
FAU - Heimark, Ronald L
AU  - Heimark RL
FAU - Guzman, Roberto
AU  - Guzman R
FAU - Baygents, James C
AU  - Baygents JC
FAU - Zohar, Yitshak
AU  - Zohar Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060706
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 9012-36-6 (Sepharose)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.81 (agarase)
SB  - IM
MH  - Animals
MH  - Antigens/chemistry
MH  - Fluorescent Antibody Technique
MH  - Glycoside Hydrolases/*chemistry
MH  - Humans
MH  - Immunoglobulin G/*chemistry
MH  - Mice
MH  - *Microfluidic Analytical Techniques
MH  - Microscopy, Fluorescence
MH  - Sepharose/*chemistry
EDAT- 2006/07/29 09:00
MHDA- 2007/05/10 09:00
CRDT- 2006/07/29 09:00
PHST- 2006/07/29 09:00 [pubmed]
PHST- 2007/05/10 09:00 [medline]
PHST- 2006/07/29 09:00 [entrez]
AID - 10.1039/b603095e [doi]
PST - ppublish
SO  - Lab Chip. 2006 Aug;6(8):1080-5. doi: 10.1039/b603095e. Epub 2006 Jul 6.