PMID- 16875581
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20060731
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 27
IP  - 5
DP  - 2006 May
TI  - [The value of multiplex fluorescence in situ hybridization in the detection of 
      complex karyotypic abnormalities of acute myeloid leukemia].
PG  - 318-22
AB  - OBJECTIVE: To investigate the value of multiplex fluorescence in situ 
      hybridization (FISH) in the detection of complex karyotypic abnormalities of 
      acute myeloid leukemia (AML). METHODS: Multiplex FISH was used in combination 
      with conventional cytogenetics (CC) and interphase FISH to study 14 cases of AML 
      with complex karyotypic abnormalities. RESULTS: In the 14 cases of AML studied, 
      conventional cytogenetics detected 23 numerical and 56 structural chromosome 
      abnormalities. Among them 4 gained whole chromosome and 4 lost whole chromosome 
      which were confirmed by multiplex FISH. Twelve chromosome losses detected by CC 
      were revised as derivative chromosomes resulted from various structural 
      aberrations, and 26 derivative and 19 marker chromosomes were characterized 
      precisely by multiplex FISH. Most of them were resulted from unbalanced 
      translocations, including 2 complex 8; 21 translocations, which have not been 
      reported previously: t (8; 21), der (8) t (8; 21) (8pter --> 8q22::21q22 --> 
      21qter), der (21) t (8; 21; 8) (8qter --> 8q22:: 21p13 --> 21q22::8q22 --> 8qter) 
      and t (21; 8; 18; 1), der (8) t (8; 21) (8pter --> 8q22:: 21q22 --> 21qter), der 
      (21) t (21; 8; 18; 1) (21p13 --> 21q22?::8q22 --> 8q24 ?:: 18??::1q??q??). The 
      complex karyotypic abnormalities involved nearly all chromosomes, of which the 
      chromosomes 17, 7 and 5 were more involved than the rest. CONCLUSION: Multiplex 
      FISH in combination with conventional cytogenetics may characterize the complex 
      chromosomal abnormalities more precisely. Introduction of this technique to the 
      study of AML with complex chromosomal abnormalities is warranted.
FAU - Ma, Li
AU  - Ma L
AD  - Department of Hematology, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing 210029, China.
FAU - Li, Jian-yong
AU  - Li JY
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Xiao, Bing
AU  - Xiao B
FAU - Qian, Si-xuan
AU  - Qian SX
FAU - Chen, Li-juan
AU  - Chen LJ
FAU - Qiu, Hai-rong
AU  - Qiu HR
FAU - Wen, Bing-zhao
AU  - Wen BZ
FAU - Xue, Yong-quan
AU  - Xue YQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Female
MH  - Humans
MH  - Leukemia, Myeloid/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Spectral Karyotyping/*methods
MH  - Translocation, Genetic
MH  - Young Adult
EDAT- 2006/08/01 09:00
MHDA- 2009/01/27 09:00
CRDT- 2006/08/01 09:00
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2006 May;27(5):318-22.