PMID- 16875599
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20131121
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 28
IP  - 3
DP  - 2006 Mar
TI  - [Bcl-XL antisense oligodeoxynucleotide sensitizes human esophageal cancer cell 
      line to 5-fluorouracil].
PG  - 173-7
AB  - OBJECTIVE: To investigate the effects of the Bcl-XL antisense 
      oligodeoxynucleotides (ASODN) in suppressing the Bcl-XL expression and increasing 
      the sensitivity of esophageal cancer cell line EC9706 to 5-fluorouracil (5-Fu). 
      METHODS: The proliferation inhibitory rate of EC9706 was assessed by MTT, the 
      expression of Bcl-XL was detected by RT-PCR and Western blot, and the apoptotic 
      changes were examined by acridine orange (AO) fluorescent staining and flow 
      cytometry, respectively. RESULTS: In the group of ASODN combined with 5-Fu, the 
      proliferation inhibitory rate of esophageal cancer cells was 71.58%, the 
      expression inhibitory rate of Bcl-XL mRNA was 81.25%, the expression of Bcl-XL 
      protein was decreased significantly. The apoptosis rates detected by AO 
      fluorescent staining and flow cytometry were 69.5% and (63.32 +/- 9.23)%, 
      respectively. There were significant differences as compared with the cell 
      control group, the vacuity control group, the N-ODN group, the ASODN group and 
      the 5-Fu group, respectively (P < 0.05). CONCLUSION: Bcl-XL ASODN combined with 
      5-Fu can effectively inhibit the proliferation of esophageal cancer cells in 
      vitro. Bcl-XL ASODN can significantly increase the sensitivity of esophageal 
      cancer cells to 5-Fu through suppressing the expression of Bcl-XL.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Henan Key Laboratory of Tumor Pathology, Department of Oncology, the First 
      Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
FAU - Wen, Hong-tao
AU  - Wen HT
FAU - Wang, Liu-xing
AU  - Wang LX
FAU - Zhang, Lan
AU  - Zhang L
FAU - Zhang, Yun-han
AU  - Zhang YH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-X Protein)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Antimetabolites, Antineoplastic/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Drug Resistance, Neoplasm
MH  - Esophageal Neoplasms/metabolism/*pathology
MH  - Fluorouracil/*pharmacology
MH  - Humans
MH  - Oligodeoxyribonucleotides, Antisense/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Transfection
MH  - bcl-X Protein/biosynthesis/*genetics
EDAT- 2006/08/01 09:00
MHDA- 2007/12/28 09:00
CRDT- 2006/08/01 09:00
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2006 Mar;28(3):173-7.