PMID- 16875745
OWN - NLM
STAT- MEDLINE
DCOM- 20070507
LR  - 20161124
IS  - 1874-1754 (Electronic)
IS  - 0167-5273 (Linking)
VI  - 117
IP  - 2
DP  - 2007 Apr 25
TI  - Therapeutic effects of concurrent autologous bone marrow cell infusion and 
      metabolic intervention in ischemia-induced angiogenesis in the 
      hypercholesterolemic mouse hindlimb.
PG  - 238-43
AB  - Lower-limb ischemia is a major health problem especially when associated to 
      hypercholesterolemia. Because of the absence of effective treatment in the 
      advanced stages of the disease, amputation is undertaken to alleviate unbearable 
      symptoms. Since tissue ischemia and hypercholesterolemia are associated with an 
      overwhelming generation of oxygen radicals, metabolic intervention with 
      antioxidants and l-arginine can induce beneficial effects beyond those achieved 
      by a novel therapeutic approach represented by the use of autologous bone marrow 
      cells (BMCs). The protective effect of BMCs and vascular protection by metabolic 
      cotreatment (1.0% vitamin E added to the chow, 0.05% vitamin C and 6% l-arginine 
      added to the drinking water) were examined in ischemia-induced angiogenesis in 
      the hypercholesterolemic mouse hindlimb. Intravenous BMC therapy improved blood 
      flow and increased capillary densities. This beneficial effect was amplified by 
      metabolic cotreatment, an intervention inducing vascular protection, at least in 
      part, through the nitric oxide pathway, reduction of systemic oxidative stress 
      and macrophage activation.
FAU - de Nigris, Filomena
AU  - de Nigris F
AD  - Department of General Pathology, Division of Clinical Pathology and Excellence 
      Research Center on Cardiovascular Diseases, 1st School of Medicine, II University 
      of Naples, Naples 80138, Italy.
FAU - Williams-Ignarro, Sharon
AU  - Williams-Ignarro S
FAU - Sica, Vincenzo
AU  - Sica V
FAU - D'Armiento, Francesco P
AU  - D'Armiento FP
FAU - Lerman, Lilach O
AU  - Lerman LO
FAU - Byrns, Russell E
AU  - Byrns RE
FAU - Sica, Giacomo
AU  - Sica G
FAU - Fiorito, Carmela
AU  - Fiorito C
FAU - Ignarro, Louis J
AU  - Ignarro LJ
FAU - Napoli, Claudio
AU  - Napoli C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060727
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
RN  - 0 (Antioxidants)
RN  - 0 (Apolipoproteins E)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apolipoproteins E/genetics
MH  - Arginine/*pharmacology
MH  - *Bone Marrow Transplantation
MH  - Combined Modality Therapy
MH  - Disease Models, Animal
MH  - Hindlimb/blood supply
MH  - Hypercholesterolemia/*complications
MH  - Ischemia/diagnostic imaging/*drug therapy
MH  - Laser-Doppler Flowmetry
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Neovascularization, Physiologic/drug effects
MH  - Nitric Oxide/metabolism
MH  - Oxidative Stress/drug effects
MH  - Peripheral Vascular Diseases/diagnostic imaging/drug therapy
MH  - Regional Blood Flow
MH  - Transplantation, Autologous
MH  - Ultrasonography
EDAT- 2006/08/01 09:00
MHDA- 2007/05/08 09:00
CRDT- 2006/08/01 09:00
PHST- 2006/02/08 00:00 [received]
PHST- 2006/05/03 00:00 [accepted]
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2007/05/08 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
AID - S0167-5273(06)00554-7 [pii]
AID - 10.1016/j.ijcard.2006.05.020 [doi]
PST - ppublish
SO  - Int J Cardiol. 2007 Apr 25;117(2):238-43. doi: 10.1016/j.ijcard.2006.05.020. Epub 
      2006 Jul 27.