PMID- 16876358
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20060918
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 162
IP  - 1-3
DP  - 2006 Oct 16
TI  - A high-throughput urinalysis of abused drugs based on a SPE-LC-MS/MS method 
      coupled with an in-house developed post-analysis data treatment system.
PG  - 95-107
AB  - A rapid urinalysis system based on SPE-LC-MS/MS with an in-house post-analysis 
      data management system has been developed for the simultaneous identification and 
      semi-quantitation of opiates (morphine, codeine), methadone, amphetamines 
      (amphetamine, methylamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 
      3,4-methylenedioxymethamphetamine (MDMA)), 11-benzodiazepines or their 
      metabolites and ketamine. The urine samples are subjected to automated solid 
      phase extraction prior to analysis by LC-MS (Finnigan Surveyor LC connected to a 
      Finnigan LCQ Advantage) fitted with an Alltech Rocket Platinum EPS C-18 column. 
      With a single point calibration at the cut-off concentration for each analyte, 
      simultaneous identification and semi-quantitation for the above mentioned drugs 
      can be achieved in a 10 min run per urine sample. A computer macro-program 
      package was developed to automatically retrieve appropriate data from the 
      analytical data files, compare results with preset values (such as cut-off 
      concentrations, MS matching scores) of each drug being analyzed and generate 
      user-defined Excel reports to indicate all positive and negative results in 
      batch-wise manner for ease of checking. The final analytical results are 
      automatically copied into an Access database for report generation purposes. 
      Through the use of automation in sample preparation, simultaneous identification 
      and semi-quantitation by LC-MS/MS and a tailored made post-analysis data 
      management system, this new urinalysis system significantly improves the quality 
      of results, reduces the post-data treatment time, error due to data transfer and 
      is suitable for high-throughput laboratory in batch-wise operation.
FAU - Cheng, Wing-Chi
AU  - Cheng WC
AD  - Forensic Science Division, Government Laboratory, 88 Chung Hau Street, Homantin 
      Government Offices, Homantin, Hong Kong SAR, China. wccheng@govtlab.gov.hk
FAU - Yau, Tsan-Sang
AU  - Yau TS
FAU - Wong, Ming-Kei
AU  - Wong MK
FAU - Chan, Lai-Ping
AU  - Chan LP
FAU - Mok, Vincent King-Kuen
AU  - Mok VK
LA  - eng
PT  - Journal Article
DEP - 20060728
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Narcotics)
SB  - IM
MH  - *Automation
MH  - Chromatography, Liquid/*methods
MH  - Forensic Medicine/methods
MH  - Humans
MH  - Narcotics/*urine
MH  - Software
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Substance Abuse Detection/*methods
MH  - Substance-Related Disorders/urine
MH  - Urinalysis
EDAT- 2006/08/01 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/01 09:00
PHST- 2005/11/19 00:00 [received]
PHST- 2006/04/14 00:00 [revised]
PHST- 2006/04/14 00:00 [accepted]
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
AID - S0379-0738(06)00395-1 [pii]
AID - 10.1016/j.forsciint.2006.04.019 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2006 Oct 16;162(1-3):95-107. doi: 
      10.1016/j.forsciint.2006.04.019. Epub 2006 Jul 28.