PMID- 16876812
OWN - NLM
STAT- MEDLINE
DCOM- 20070611
LR  - 20131121
IS  - 0021-9797 (Print)
IS  - 0021-9797 (Linking)
VI  - 302
IP  - 2
DP  - 2006 Oct 15
TI  - Adsorption behavior and activity of hexokinase.
PG  - 417-23
AB  - The study on the adsorption of hexokinase (HK) onto silicon wafers was carried 
      out by means of in situ ellipsometry and atomic force microscopy in the liquid. 
      The thickness values of the adsorbed HK layer determined by both techniques were 
      in excellent agreement and evidenced HK monolayer formation. The adsorption of HK 
      onto Si wafers was favored at low ionic strength, indicating that the adsorption 
      is mainly driven by electrostatic forces, since salt screens not only the 
      segment-segment repulsion but also the segment-surface attraction when the salt 
      concentration increases. The enzymatic activity of free HK and of adsorbed HK was 
      measured as a function of time. Free HK in solution lost activity upon storage. 
      Contrarily, adsorbed HK kept its activity level even after 48 h storage at room 
      temperature. This outstanding behavior was attributed to specific orientation of 
      the HK active site to the solution.
FAU - Pancera, S M
AU  - Pancera SM
AD  - Institut fur Nanotechnologie, Forschungszentrum Karlsruhe GmbH, D-76021 
      Karlsruhe, Germany.
FAU - Gliemann, H
AU  - Gliemann H
FAU - Schimmel, Th
AU  - Schimmel T
FAU - Petri, D F S
AU  - Petri DF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060731
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - EC 2.7.1.1 (Hexokinase)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Adsorption
MH  - Hexokinase/*chemistry
MH  - Microscopy, Atomic Force/methods
MH  - Particle Size
MH  - Sensitivity and Specificity
MH  - Silicon/chemistry
MH  - Spectrophotometry, Ultraviolet/methods
MH  - Surface Properties
MH  - Time Factors
EDAT- 2006/08/01 09:00
MHDA- 2007/06/15 09:00
CRDT- 2006/08/01 09:00
PHST- 2006/04/11 00:00 [received]
PHST- 2006/06/09 00:00 [revised]
PHST- 2006/06/29 00:00 [accepted]
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
AID - S0021-9797(06)00583-2 [pii]
AID - 10.1016/j.jcis.2006.06.066 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2006 Oct 15;302(2):417-23. doi: 
      10.1016/j.jcis.2006.06.066. Epub 2006 Jul 31.