PMID- 16877804
OWN - NLM
STAT- MEDLINE
DCOM- 20061120
LR  - 20181113
IS  - 1234-1983 (Print)
IS  - 1234-1983 (Linking)
VI  - 47
IP  - 3
DP  - 2006
TI  - Loop formation by the transgene WAP:6xHishGH in transgenic rabbit fibroblasts, 
      revealed by fluorescence in situ hybridization to nuclear halos.
PG  - 247-9
AB  - Using fluorescence in situ hybridization (FISH) to somatic nuclear halos from 
      transgenic rabbits WAP:6xHishGH, we present evidence for stability of 
      transgenesis at the chromatin level. FISH performed on fibroblasts from a 
      homozygous individual showed 2 independent loops from both chromosomes of pair 7. 
      On a heterozygous individual, FISH detected a single loop. According to the 
      concept of chromatin loops and their influence on gene expression, this shows 
      that the human growth hormone transgene, which was actively expressed in mammary 
      gland under the influence of the tissue-specific promoter, was inactive in 
      examined skin fibroblasts.
FAU - Michalak, Ewa
AU  - Michalak E
AD  - Department of Biochemistry and Biotechnology, Agricultural University, Poznan, 
      Poland. elenis@wp.pl
FAU - Lipinski, Daniel
AU  - Lipinski D
FAU - Slomski, Ryszard
AU  - Slomski R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (Chromatin)
RN  - 12629-01-5 (Human Growth Hormone)
SB  - IM
MH  - Animals
MH  - Chromatin/*genetics
MH  - Fibroblasts
MH  - Human Growth Hormone/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Rabbits/*genetics
MH  - Transgenes/*genetics
EDAT- 2006/08/01 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/01 09:00
PHST- 2006/08/01 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/01 09:00 [entrez]
AID - 347 [pii]
AID - 10.1007/BF03194631 [doi]
PST - ppublish
SO  - J Appl Genet. 2006;47(3):247-9. doi: 10.1007/BF03194631.