PMID- 16879245
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 145
IP  - 2
DP  - 2006 Aug
TI  - Rotavirus-specific T cell responses and cytokine mRNA expression in children with 
      diabetes-associated autoantibodies and type 1 diabetes.
PG  - 261-70
AB  - Rotavirus infections have been implicated as a possible trigger of type 1 
      diabetes. We elucidated this connection by comparing peripheral blood T cell 
      responses to rotavirus between children with newly diagnosed type 1 diabetes (n = 
      43), healthy children with multiple diabetes-associated autoantibodies (n = 36) 
      and control children carrying human leukocyte antigen (HLA)-conferred 
      susceptibility to type 1 diabetes but without autoantibodies (n = 104). 
      Lymphocyte proliferation assays based on stimulation with an antigen were 
      performed using freshly isolated peripheral blood mononuclear cells (PBMC) and 
      IgG and IgA class rotavirus antibodies were measured using plasma samples 
      collected from the children. The expression of interferon (IFN)-gamma, 
      interleukin (IL)-4, IL-10 and transforming growth factor (TGF)-beta in PBMC was 
      studied with real-time polymerase chain reaction (PCR) in a subgroup of 38 
      children. No differences were observed in the strength or frequency of positive T 
      cell responses to rotavirus between children with overt diabetes, children with 
      multiple autoantibodies and control children. Children with diabetes-associated 
      autoantibodies had, instead, stronger T cell responses to purified coxsackie B4 
      virus than control children. Rotavirus-stimulated lymphocytes from 
      autoantibody-positive children produced more IL-4 and phytohaemagglutinin 
      (PHA)-stimulated lymphocytes more IL-4 and IFN-gamma than lymphocytes from 
      control children. PHA-stimulated lymphocytes from children with diabetes also 
      produced more IL-4 and purified protein derivative (PPD)-stimulated lymphocytes 
      less TGF-beta than lymphocytes from autoantibody-negative control children. In 
      conclusion, our lymphocyte proliferation studies did not provide evidence 
      supporting an association between rotavirus infections and the development of 
      type 1 diabetes or diabetes-associated autoantibodies in young children.
FAU - Makela, M
AU  - Makela M
AD  - Immunogenetics Laboratory, University of Turku, Medicity, Biocity 4. krs, 
      Tykistokatu 6A, 20520 Turku, Finland. miia.makela@utu.fi
FAU - Oling, V
AU  - Oling V
FAU - Marttila, J
AU  - Marttila J
FAU - Waris, M
AU  - Waris M
FAU - Knip, M
AU  - Knip M
FAU - Simell, O
AU  - Simell O
FAU - Ilonen, J
AU  - Ilonen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antigens, Viral)
RN  - 0 (Autoantibodies)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - Autoantibodies/*immunology
MH  - Case-Control Studies
MH  - Cell Proliferation
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/*genetics
MH  - Diabetes Mellitus, Type 1/*immunology
MH  - Female
MH  - Humans
MH  - Insulin-Secreting Cells/immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-4/immunology
MH  - Linear Models
MH  - Lymphocyte Activation
MH  - Male
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rotavirus/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Transforming Growth Factor beta/immunology
PMC - PMC1809689
EDAT- 2006/08/02 09:00
MHDA- 2006/09/22 09:00
PMCR- 2007/08/01
CRDT- 2006/08/02 09:00
PHST- 2006/08/02 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/08/02 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - CEI3146 [pii]
AID - 10.1111/j.1365-2249.2006.03146.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2006 Aug;145(2):261-70. doi: 10.1111/j.1365-2249.2006.03146.x.