PMID- 16879255
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 145
IP  - 2
DP  - 2006 Aug
TI  - Arthritogenicity of collagen type II is increased by chlorination.
PG  - 339-45
AB  - During inflammation, activated neutrophils, monocytes and macrophages produce and 
      release myeloperoxidase (MPO). MPO converts hydrogen peroxide to hypochlorous 
      acid, a highly reactive and oxidizing agent. Proteins subjected to hypochlorous 
      acid become chlorinated. We analysed how chlorination of the cartilage antigen 
      collagen type II (CII) affects its immunogenic and arthritogenic properties by 
      studying immune responses to chlorinated CII in comparison to immune responses to 
      CII and by studying the development of arthritis in rats immunized with CII-Cl. 
      CII-Cl immunization of LEW.1AV1 rats caused a 100% incidence of arthritis with a 
      mean maximum score of 9.2 (maximal score possible 16). The same dose of 
      non-chlorinated CII did not induce arthritis at all. Rats immunized with CII-Cl 
      developed high anti-CII-Cl IgG titres and also developed IgG antibodies 
      recognizing the non-chlorinated form of CII. Analysis of cytokine mRNA expression 
      in lymph nodes 10 days after immunzation revealed an increased expression of 
      interferon (IFN)-gamma mRNA and interleukin (IL)-1beta mRNA in CII-Cl-immunized 
      rats compared to CII-immunized rats. Thus, chlorination of CII increased its 
      immunogenicity as well as its arthritogenicity. As neutrophils, monocytes and 
      macrophages are abundant cells in arthritic joints of patients with rheumatoid 
      arthritis, chlorination might be a mechanism by which immunoreactivity to CII is 
      induced and by which chronic joint inflammation is supported.
FAU - Westman, E
AU  - Westman E
AD  - Department of Medicine, Rheumatology unit, Karolinska Institutet, S-171 76 
      Stockholm, Sweden.
FAU - Lundberg, K
AU  - Lundberg K
FAU - Erlandsson Harris, H
AU  - Erlandsson Harris H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Autoantibodies)
RN  - 0 (Chlorides)
RN  - 0 (Collagen Type II)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 712K4CDC10 (Hypochlorous Acid)
RN  - 82115-62-6 (Interferon-gamma)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Arthritis/*chemically induced/immunology/metabolism
MH  - Autoantibodies/analysis
MH  - Chlorides/metabolism
MH  - Collagen Type II/*adverse effects/immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Hydrogen Peroxide/metabolism
MH  - Hypochlorous Acid/metabolism
MH  - Immunoglobulin G/analysis
MH  - Interferon-gamma/genetics/immunology
MH  - Interleukin-1/genetics/immunology
MH  - Lymph Nodes/immunology
MH  - Peroxidase/metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Recombinant Proteins/immunology
PMC - PMC1809685
EDAT- 2006/08/02 09:00
MHDA- 2006/09/22 09:00
PMCR- 2007/08/01
CRDT- 2006/08/02 09:00
PHST- 2006/08/02 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/08/02 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - CEI3129 [pii]
AID - 10.1111/j.1365-2249.2006.03129.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2006 Aug;145(2):339-45. doi: 10.1111/j.1365-2249.2006.03129.x.