PMID- 16882262
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20061115
IS  - 1341-8076 (Print)
IS  - 1341-8076 (Linking)
VI  - 32
IP  - 4
DP  - 2006 Aug
TI  - Reduction of aquaporin-8 on fetal membranes under oligohydramnios in mice lacking 
      prostaglandin F2 alpha receptor.
PG  - 373-8
AB  - AIM: To investigate the association between aquaporin-8 (AQP-8: a water channel 
      protein) expression in fetal membranes and oligohydramnios during near-term and 
      postdate pregnancy, we set up an oligohydramnios model using prostaglandin F2 
      alpha receptor (FP)-deficient mice. METHODS: Pregnant FP-deficient mice from 14 
      to 21 gestational days (GD) were killed to measure the amniotic fluid volume 
      (AFV), and fetal membranes were collected for the analysis of aquaporin-8 
      expression. RESULTS: The AFV was highest at 14 GD, and was significantly 
      decreased to 28% and 0% at 20 GD and 21 GD, respectively, compared with the 
      volume at 14 GD. Immunohistochemistry and immunoblot analysis showed that 
      aquaporin-8 was expressed in the basal component of fetal membranes, and that the 
      protein level was significantly decreased to 60% at 20 GD compared with that at 
      14 GD. CONCLUSIONS: We demonstrated that AQP-8 expression in the fetal membrane 
      was decreased at post term in FP-deficient mice. Our findings suggest that 
      aquaporin-8 in fetal membranes may be involved in the regulation of AFV, 
      especially when oligohydramnios occurs.
FAU - Shioji, Mitsunori
AU  - Shioji M
AD  - Department of Obstetrics and Gynecology, Osaka University, Graduate School of 
      Medicine, Osaka, Japan.
FAU - Fukuda, Hirotsugu
AU  - Fukuda H
FAU - Kanzaki, Toru
AU  - Kanzaki T
FAU - Wasada, Kenshi
AU  - Wasada K
FAU - Kanagawa, Takeshi
AU  - Kanagawa T
FAU - Shimoya, Koichiro
AU  - Shimoya K
FAU - Mu, Junwu
AU  - Mu J
FAU - Sugimoto, Yukihiko
AU  - Sugimoto Y
FAU - Murata, Yuji
AU  - Murata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Obstet Gynaecol Res
JT  - The journal of obstetrics and gynaecology research
JID - 9612761
RN  - 0 (Aquaporins)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (aquaporin 8)
RN  - 0 (prostaglandin F2alpha receptor)
SB  - IM
MH  - Amniotic Fluid/physiology
MH  - Animals
MH  - Aquaporins/*biosynthesis
MH  - Extraembryonic Membranes/*metabolism
MH  - Female
MH  - Mice
MH  - Mice, Knockout
MH  - Oligohydramnios/*metabolism
MH  - Pregnancy
MH  - Receptors, Prostaglandin/*metabolism
EDAT- 2006/08/03 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/08/03 09:00
PHST- 2006/08/03 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/08/03 09:00 [entrez]
AID - JOG425 [pii]
AID - 10.1111/j.1447-0756.2006.00425.x [doi]
PST - ppublish
SO  - J Obstet Gynaecol Res. 2006 Aug;32(4):373-8. doi: 
      10.1111/j.1447-0756.2006.00425.x.