PMID- 16882887
OWN - NLM
STAT- MEDLINE
DCOM- 20061221
LR  - 20220129
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Linking)
VI  - 27
IP  - 3
DP  - 2006 Nov 27
TI  - Coordinated multitissue transcriptional and plasma metabonomic profiles following 
      acute caloric restriction in mice.
PG  - 187-200
AB  - Caloric restriction (CR) increases healthy life span in a range of organisms. The 
      underlying mechanisms are not understood but appear to include changes in gene 
      expression, protein function, and metabolism. Recent studies demonstrate that 
      acute CR alters mortality rates within days in flies. Multitissue transcriptional 
      changes and concomitant metabolic responses to acute CR have not been described. 
      We generated whole genome RNA transcript profiles in liver, skeletal muscle, 
      colon, and hypothalamus and simultaneously measured plasma metabolites using 
      proton nuclear magnetic resonance in mice subjected to acute CR. Liver and muscle 
      showed increased gene expressions associated with fatty acid metabolism and a 
      reduction in those involved in hepatic lipid biosynthesis. Glucogenic amino acids 
      increased in plasma, and gene expression for hepatic gluconeogenesis was 
      enhanced. Increased expression of genes for hormone-mediated signaling and 
      decreased expression of genes involved in protein binding and development 
      occurred in hypothalamus. Cell proliferation genes were decreased and cellular 
      transport genes increased in colon. Acute CR captured many, but not all, hepatic 
      transcriptional changes of long-term CR. Our findings demonstrate a clear 
      transcriptional response across multiple tissues during acute CR, with congruent 
      plasma metabolite changes. Liver and muscle switched gene expression away from 
      energetically expensive biosynthetic processes toward energy conservation and 
      utilization processes, including fatty acid metabolism and gluconeogenesis. Both 
      muscle and colon switched gene expression away from cellular proliferation. Mice 
      undergoing acute CR rapidly adopt many transcriptional and metabolic changes of 
      long-term CR, suggesting that the beneficial effects of CR may require only a 
      short-term reduction in caloric intake.
FAU - Selman, Colin
AU  - Selman C
AD  - Centre for Diabetes and Endocrinology, Department of Medicine, University College 
      London, Rayne Institute, London, United Kingdom. c.selman@ucl.ac.uk
FAU - Kerrison, Nicola D
AU  - Kerrison ND
FAU - Cooray, Anisha
AU  - Cooray A
FAU - Piper, Matthew D W
AU  - Piper MD
FAU - Lingard, Steven J
AU  - Lingard SJ
FAU - Barton, Richard H
AU  - Barton RH
FAU - Schuster, Eugene F
AU  - Schuster EF
FAU - Blanc, Eric
AU  - Blanc E
FAU - Gems, David
AU  - Gems D
FAU - Nicholson, Jeremy K
AU  - Nicholson JK
FAU - Thornton, Janet M
AU  - Thornton JM
FAU - Partridge, Linda
AU  - Partridge L
FAU - Withers, Dominic J
AU  - Withers DJ
LA  - eng
GR  - SF19106/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060801
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Animals
MH  - Blood Chemical Analysis
MH  - *Caloric Restriction
MH  - Colon/*metabolism
MH  - Down-Regulation
MH  - Energy Intake
MH  - Fatty Acids/metabolism
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Hypothalamus/*metabolism
MH  - Lipid Metabolism
MH  - Liver/*metabolism
MH  - Longevity/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*metabolism
MH  - Organ Specificity
MH  - Specific Pathogen-Free Organisms
MH  - *Transcription, Genetic
MH  - Up-Regulation
EDAT- 2006/08/03 09:00
MHDA- 2006/12/22 09:00
CRDT- 2006/08/03 09:00
PHST- 2006/08/03 09:00 [pubmed]
PHST- 2006/12/22 09:00 [medline]
PHST- 2006/08/03 09:00 [entrez]
AID - 00084.2006 [pii]
AID - 10.1152/physiolgenomics.00084.2006 [doi]
PST - ppublish
SO  - Physiol Genomics. 2006 Nov 27;27(3):187-200. doi: 
      10.1152/physiolgenomics.00084.2006. Epub 2006 Aug 1.