PMID- 16882985
OWN - NLM
STAT- MEDLINE
DCOM- 20060925
LR  - 20181113
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 20
IP  - 15
DP  - 2006 Aug 1
TI  - Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in 
      concert to ensure homeostatic maintenance of the Escherichia coli chromosome.
PG  - 2121-34
AB  - Initiation of DNA replication in Eschericia coli requires the ATP-bound form of 
      the DnaA protein. The conversion of DnaA-ATP to DnaA-ADP is facilitated by a 
      complex of DnaA, Hda (homologous to DnaA), and DNA-loaded beta-clamp proteins in 
      a process termed RIDA (regulatory inactivation of DnaA). Hda-deficient cells 
      initiate replication at each origin mainly once per cell cycle, and the rare 
      reinitiation events never coincide with the end of the origin sequestration 
      period. Therefore, RIDA is not the predominant mechanism to prevent immediate 
      reinitiation from oriC. The cellular level of Hda correlated directly with dnaA 
      gene expression such that Hda deficiency led to reduced dnaA gene expression, and 
      overproduction of Hda led to DnaA overproduction. Hda-deficient cells were very 
      sensitive to variations in the cellular level of DnaA, and DnaA overproduction 
      led to uncontrolled initiation of replication from oriC, causing severe growth 
      retardation or cell death. Based on these observations, we propose that both RIDA 
      and dnaA gene autoregulation are required as homeostatic mechanisms to ensure 
      that initiation of replication occurs at the same time relative to cell mass in 
      each cell cycle.
FAU - Riber, Leise
AU  - Riber L
AD  - Department of Life Sciences and Chemistry, Roskilde University, Denmark.
FAU - Olsson, Jan A
AU  - Olsson JA
FAU - Jensen, Rasmus B
AU  - Jensen RB
FAU - Skovgaard, Ole
AU  - Skovgaard O
FAU - Dasgupta, Santanu
AU  - Dasgupta S
FAU - Marinus, Martin G
AU  - Marinus MG
FAU - Lobner-Olesen, Anders
AU  - Lobner-Olesen A
LA  - eng
GR  - R01 GM063790/GM/NIGMS NIH HHS/United States
GR  - R01 GM063790-04/GM/NIGMS NIH HHS/United States
GR  - GM63790/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DnaA protein, Bacteria)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Trans-Activators)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (Hda protein, E coli)
SB  - IM
MH  - Adenosine Triphosphatases/*metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Cell Death
MH  - *Chromosomes, Bacterial
MH  - DNA Replication
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - *Escherichia coli/genetics/metabolism
MH  - Escherichia coli Proteins/*metabolism
MH  - *Gene Expression Regulation, Bacterial
MH  - *Homeostasis
MH  - Replication Origin
MH  - Trans-Activators
PMC - PMC1536062
EDAT- 2006/08/03 09:00
MHDA- 2006/09/26 09:00
PMCR- 2007/02/01
CRDT- 2006/08/03 09:00
PHST- 2006/08/03 09:00 [pubmed]
PHST- 2006/09/26 09:00 [medline]
PHST- 2006/08/03 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 20/15/2121 [pii]
AID - 10.1101/gad.379506 [doi]
PST - ppublish
SO  - Genes Dev. 2006 Aug 1;20(15):2121-34. doi: 10.1101/gad.379506.