PMID- 16883624
OWN - NLM
STAT- MEDLINE
DCOM- 20080117
LR  - 20151119
IS  - 0263-6484 (Print)
IS  - 0263-6484 (Linking)
VI  - 25
IP  - 6
DP  - 2007 Nov-Dec
TI  - Protective effect of L-carnitine on testicular ischaemia-reperfusion injury in 
      rats.
PG  - 611-8
AB  - Testicular torsion is a urological emergency referred to as 'acute scrotum', 
      because inappropriate treatment can lead to male subfertility and infertility. A 
      possible cause of testicular damage is the ischaemia-reperfusion (I/R) injury 
      attributed to oxygen free radicals. L-carnitine, a vitamin-like antioxidant, 
      plays a pivotal role in the maturation of spermatozoa within the reproductive 
      tract. The aim of the present paper was to determine the protective effect of 
      L-carnitine on testicular I/R-induced injury. Thirty-two male rats were divided 
      into 4 groups (n = 8). Testicular torsion was created by rotating the right 
      testis 720 degrees in a clockwise direction. Group 1: sham-operated control; 
      group 2: ischaemia; group 3: I/R; group 4: ischaemia-L-carnitine 
      treatment-reperfusion group. L-carnitine (500 mg kg(-1), intraperitoneally) was 
      administered before 30 min of detorsion in Group 4. After torsion (5 h) and 
      detorsion (5 h), bilateral orchidectomy was performed. The malondialdehyde (MDA) 
      level was evaluated in testes. Histopathologically, Johnsen's spermatogenesis 
      criteria and mean seminiferous tubule diameter (MSTD) measurements were used. 
      Testicular MDA levels were higher in the torsion group compared to the 
      sham-control group (p < 0.05). Detorsion (reperfusion) caused a further increase 
      in MDA levels (p < 0.05). Pretreatment with L-carnitine prevented a further 
      increase in MDA levels (p < 0.05). Histologically, torsion caused some separation 
      among germinal cells in the seminiferous tubules, which became much more 
      prominent in the I/R group but was attenuated with L-carnitine pretreatment. In 
      conclusion, L-carnitine pretreatment may have a protective effect in experimental 
      testicular torsion-detorsion model in rats by its well-known antioxidant 
      potential.
CI  - Copyright (c) 2006 John Wiley & Sons, Ltd.
FAU - Dokmeci, Dikmen
AU  - Dokmeci D
AD  - Department of Pharmacology, Faculty of Medicine, Trakya University, Edirne, 
      Turkey. ddokmeci@trakya.edu.tr
FAU - Inan, Mustafa
AU  - Inan M
FAU - Basaran, Umit Nusret
AU  - Basaran UN
FAU - Yalcin, Omer
AU  - Yalcin O
FAU - Aydogdu, Nurettin
AU  - Aydogdu N
FAU - Turan, Fatma Nesrin
AU  - Turan FN
FAU - Uz, Yesim Hulya
AU  - Uz YH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 12001-76-2 (Vitamin B Complex)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Animals
MH  - Carnitine/administration & dosage/*pharmacology
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Orchiectomy
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/complications/*physiopathology
MH  - Seminiferous Tubules/blood supply/drug effects/metabolism
MH  - Spermatic Cord Torsion/complications/prevention & control
MH  - Testis/blood supply/*drug effects/metabolism
MH  - Vitamin B Complex/administration & dosage/pharmacology
EDAT- 2006/08/03 09:00
MHDA- 2008/01/18 09:00
CRDT- 2006/08/03 09:00
PHST- 2006/08/03 09:00 [pubmed]
PHST- 2008/01/18 09:00 [medline]
PHST- 2006/08/03 09:00 [entrez]
AID - 10.1002/cbf.1355 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2007 Nov-Dec;25(6):611-8. doi: 10.1002/cbf.1355.