PMID- 16884591
OWN - NLM
STAT- MEDLINE
DCOM- 20110105
LR  - 20190513
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 44
IP  - 6
DP  - 2006 Jul
TI  - Solid-phase microextraction coupled with liquid chromatography for determination 
      of beta-carotene in food.
PG  - 354-8
AB  - Beta-carotene in vegetables and nutritional products is analyzed using 
      solid-phase microextraction (SPME) coupled with liquid chromatography (LC) to 
      improve the speed of analysis and to reduce the consumption of organic solvents. 
      The relative standard deviations (RSDs) of this analytical method for 
      beta-carotene determinations in vegetables and nutritional products are 
      approximately 10% and 5%, respectively. The amount of beta-carotene was found to 
      vary from 0.35 +/- 0.05 ppm to 76.5 +/- 6.9 ppm for several vegetables in Taiwan. 
      This method was linear over the range of 0.4-40 ppm with correlation coefficients 
      higher than 0.997. The experimentally determined level of beta-carotene in 
      nutritional products varied from 3.8 +/- 0.2 ppm to 24.6 +/- 1.1 ppm following 
      SPME-LC. The recoveries of beta-carotene for these measurements following SPME 
      were all higher than 97% +/- 2% (n = 3). The detection limits of beta-carotene 
      for this method were from 0.027 to 0.054 ppm. Conventional solvent extractions 
      take approximately 4-6 h for extraction and reconcentration but SPME takes 
      approximately 1 h. From several tens to hundreds of milliliters, organic solvents 
      can be saved using SPME. SPME provides better analyses on beta-carotene than 
      conventional solvent extraction for nutritional products in terms of speed, 
      precision, simplicity, and solvent consumption.
FAU - Tsai, H Y
AU  - Tsai HY
AD  - School of Applied Chemistry, Chung Shan Medical University, 110, Sec 1, Chien-kuo 
      N. Road, Taichung 402, Taiwan.
FAU - Chang, C M
AU  - Chang CM
FAU - Shen, J L
AU  - Shen JL
FAU - Chen, L J
AU  - Chen LJ
FAU - Yang, T F
AU  - Yang TF
FAU - Bor Fuh, C
AU  - Bor Fuh C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 01YAE03M7J (beta Carotene)
SB  - IM
MH  - Beverages/analysis
MH  - Chromatography, Liquid/*methods
MH  - Dietary Supplements/analysis
MH  - Drug Stability
MH  - Food Analysis/*methods
MH  - Hydrogen-Ion Concentration
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Microextraction/*methods
MH  - Time Factors
MH  - Vegetables/chemistry
MH  - beta Carotene/*analysis
EDAT- 2006/08/04 09:00
MHDA- 2011/01/06 06:00
CRDT- 2006/08/04 09:00
PHST- 2006/08/04 09:00 [pubmed]
PHST- 2011/01/06 06:00 [medline]
PHST- 2006/08/04 09:00 [entrez]
AID - 10.1093/chromsci/44.6.354 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2006 Jul;44(6):354-8. doi: 10.1093/chromsci/44.6.354.