PMID- 16884756
OWN - NLM
STAT- MEDLINE
DCOM- 20061109
LR  - 20081121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 354
IP  - 1
DP  - 2006 Oct 10
TI  - Mapping of human herpesvirus 6 immediate-early 2 protein transactivation domains.
PG  - 91-102
AB  - The immediate-early 2 (IE2) protein of human herpesvirus 6 (HHV-6) is a potent 
      transactivator of multiple cellular and viral promoters. Deletion mutants of 
      HHV-6 variant A IE2 allowed us to map functional transactivation domains acting 
      on complex and minimal promoter sequences. This mapping showed that both the 
      N-terminal and C-terminal domains of IE2 are required for efficient 
      transactivation, and that deletion of the C-terminal (1397-1466) tail of IE2 
      drastically reduces both transactivation and the intranuclear distribution of 
      IE2. Moreover, we determined that the ATF/CRE binding site within the HHV-6A 
      polymerase promoter is not required for efficient transactivation by IE2, whereas 
      the R3 repeat region of the putative immediate-early promoter of HHV-6A is 
      responsive to and positively regulated by IE2. These results contrast sharply to 
      that of human cytomegalovirus (HCMV) IE2, which down-regulates its promoter. Our 
      characterization of HHV-6 IE2 transactivating activity provides a better 
      understanding of the complex interactions of this protein with the viral and 
      cellular transcription machinery and highlights significant differences with the 
      IE2 protein of HCMV.
FAU - Tomoiu, Andru
AU  - Tomoiu A
AD  - Laboratory of Virology, Rheumatology and Immunology Research Center, Centre de 
      Recherche du CHUL and Faculty of Medicine, Laval University, 2705 Laurier Blvd., 
      Room T1-49, Quebec, Qc, Canada G1V 4G2. Andru.Tomoiu@crchul.ulaval.ca
FAU - Gravel, Annie
AU  - Gravel A
FAU - Flamand, Louis
AU  - Flamand L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060801
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Immediate-Early Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Cell Line
MH  - Cell Nucleus/chemistry
MH  - Genes, Reporter
MH  - Herpesvirus 6, Human/*genetics/physiology
MH  - Humans
MH  - Immediate-Early Proteins/analysis/*chemistry/genetics/*physiology
MH  - Luciferases/biosynthesis/genetics
MH  - Microscopy, Fluorescence
MH  - Mutation
MH  - Promoter Regions, Genetic
MH  - Protein Structure, Tertiary
MH  - Sequence Deletion
MH  - *Transcriptional Activation
EDAT- 2006/08/04 09:00
MHDA- 2006/11/11 09:00
CRDT- 2006/08/04 09:00
PHST- 2006/02/28 00:00 [received]
PHST- 2006/04/10 00:00 [revised]
PHST- 2006/06/23 00:00 [accepted]
PHST- 2006/08/04 09:00 [pubmed]
PHST- 2006/11/11 09:00 [medline]
PHST- 2006/08/04 09:00 [entrez]
AID - S0042-6822(06)00433-8 [pii]
AID - 10.1016/j.virol.2006.06.030 [doi]
PST - ppublish
SO  - Virology. 2006 Oct 10;354(1):91-102. doi: 10.1016/j.virol.2006.06.030. Epub 2006 
      Aug 1.