PMID- 16884876
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20211203
IS  - 0379-0738 (Print)
IS  - 0379-0738 (Linking)
VI  - 162
IP  - 1-3
DP  - 2006 Oct 16
TI  - Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in 
      forensic medicine.
PG  - 66-73
AB  - The human leukocyte antigen (HLA) system is known to be the most complex 
      polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has 
      the second largest number of alleles. The purpose of this study is to develop an 
      oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic 
      identification, anthropology, tissue transplantation, and other genetic research 
      fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 
      1198 unrelated healthy Chinese Han individuals originating from various parts of 
      China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) 
      coupled with the oligonucleotide micro-array technology was used to detect and 
      type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, 
      consistency and specificity were evaluated for use in forensic identification. 
      Furthermore, a meta-analysis was carried out by comparing the allele frequencies 
      of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities 
      and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp 
      amplification product, with no other amplification products in this length range. 
      The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction 
      system of 25 microl. The population studied appeared to be not in Hardy-Weinberg 
      equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected 
      probability of exclusion (PE), polymorphic information content (PIC), and 
      discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic 
      group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. 
      A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and 
      *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the 
      sample. One hundred and sixteen genotypes were found. The five most frequent 
      genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), 
      *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that 
      there were uniquely distributed features of DRB1 alleles among various ethnic 
      populations and among the studied population groups from various regions with the 
      same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed 
      and established based on the oligonucleotide micro-array technology. The HLA-DRB1 
      typing of the Han population in Shanghai has revealed a relatively high 
      heterogeneity. Information obtained in this study will be useful for medical and 
      forensic applications as well as in anthropology research. Large-scale 
      micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 
      genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the 
      database of the Shanghai Han group have useful applications in processing 
      forensic casework (as personal identification, paternity test), tracing 
      population migration and genetic diagnosis.
FAU - Jiang, Bin
AU  - Jiang B
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Science, Fudan University, Shanghai, PR China.
FAU - Li, Yao
AU  - Li Y
FAU - Wu, Hai
AU  - Wu H
FAU - He, Xianmin
AU  - He X
FAU - Li, Chengtao
AU  - Li C
FAU - Li, Li
AU  - Li L
FAU - Tang, Rong
AU  - Tang R
FAU - Xie, Yi
AU  - Xie Y
FAU - Mao, Yumin
AU  - Mao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060801
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Animals
MH  - China
MH  - DNA Fingerprinting
MH  - DNA Primers
MH  - DNA Probes
MH  - Ethnicity/genetics
MH  - Gene Frequency
MH  - *Genetics, Population
MH  - Genotype
MH  - HLA-DR Antigens/blood/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Polymerase Chain Reaction
EDAT- 2006/08/04 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/04 09:00
PHST- 2006/08/04 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/04 09:00 [entrez]
AID - S0379-0738(06)00384-7 [pii]
AID - 10.1016/j.forsciint.2006.06.041 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2006 Oct 16;162(1-3):66-73. doi: 
      10.1016/j.forsciint.2006.06.041. Epub 2006 Aug 1.