PMID- 1688496
OWN - NLM
STAT- MEDLINE
DCOM- 19900209
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 75
IP  - 1
DP  - 1990 Jan 1
TI  - Granulocyte colony-stimulating factor stimulates human mature neutrophilic 
      granulocytes to produce interferon-alpha.
PG  - 17-9
AB  - Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein hormone that 
      specifically stimulates both production and functional activation of neutrophils, 
      while interferon-alpha (IFN-alpha) is known to suppress myelopoiesis, including 
      neutrophil production in vivo and in vitro. On a possibility that IFN-alpha may 
      operate as one of the inhibitory feedback factors in neutropoiesis, we examined 
      whether neutrophils produce IFN-alpha in response to G-CSF. Northern blot 
      analysis showed that messenger RNA (mRNA) for human IFN-alpha 1 became detectable 
      time-dependently in highly purified human neutrophils incubated with purified 
      recombinant human G-CSF (rhG-CSF). But such transcription was not observed either 
      in neutrophils incubated with other neutrophil activators, such as 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or lipopolysaccharides (LPS), or in 
      blood mononuclear cells incubated with rhG-CSF. In addition, radioimmunoassay for 
      human IFN-alpha showed that its levels in culture medium of the rhG-CSF-treated 
      neutrophils rose markedly (up to approximately 100 IU/mL/1 x 10(7) cells) in a 
      time-dependent way, compared with those of nonstimulated neutrophils. These 
      findings suggest that the G-CSF/IFN-alpha system may participate in the feedback 
      regulatory loop of neutropoiesis.
FAU - Shirafuji, N
AU  - Shirafuji N
AD  - Department of Hematology-Oncology, University of Tokyo, Japan.
FAU - Matsuda, S
AU  - Matsuda S
FAU - Ogura, H
AU  - Ogura H
FAU - Tani, K
AU  - Tani K
FAU - Kodo, H
AU  - Kodo H
FAU - Ozawa, K
AU  - Ozawa K
FAU - Nagata, S
AU  - Nagata S
FAU - Asano, S
AU  - Asano S
FAU - Takaku, F
AU  - Takaku F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Interferon Type I)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Blotting, Northern
MH  - Colony-Stimulating Factors/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Granulocyte Colony-Stimulating Factor
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon Type I/*biosynthesis
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins
MH  - Time Factors
MH  - Transcription, Genetic/drug effects
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - S0006-4971(20)85811-X [pii]
PST - ppublish
SO  - Blood. 1990 Jan 1;75(1):17-9.