PMID- 16885301
OWN - NLM
STAT- MEDLINE
DCOM- 20061005
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 8
DP  - 2006 Aug
TI  - Bacterioplankton community composition along a salinity gradient of sixteen 
      high-mountain lakes located on the Tibetan Plateau, China.
PG  - 5478-85
AB  - The influence of altitude and salinity on bacterioplankton community composition 
      (BCC) in 16 high-mountain lakes located at altitudes of 2,817 to 5,134 m on the 
      Eastern Qinghai-Xizang (Tibetan) Plateau, China, spanning a salinity gradient 
      from 0.02% (freshwater) to 22.3% (hypersaline), was investigated. Three different 
      methods, fluorescent in situ hybridization, denaturing gradient gel 
      electrophoresis (DGGE) with subsequent band sequencing, and reverse line blot 
      hybridization (RLB) with probes targeting 17 freshwater bacterial groups, were 
      used for analysis of BCC. Furthermore, the salt tolerances of 47 strains 
      affiliated with groups detected in or isolated from the Tibetan habitats were 
      investigated. Altitude was not found to influence BCC significantly within the 
      investigated range. Several groups of typical freshwater bacteria, e.g., the 
      ACK-M1 cluster and the Polynucleobacter group, were detected in habitats located 
      above 4,400 m. Salinity was found to be the dominating environmental factor 
      controlling BCC in the investigated lakes, resulting in only small overlaps in 
      the BCCs of freshwater and hypersaline lakes. The relative abundances of 
      different classes of Proteobacteria showed a sharp succession along the salinity 
      gradient. Both DGGE and RLB demonstrated that a few freshwater bacterial groups, 
      e.g., GKS98 and LD2, appeared over wide salinity ranges. Six freshwater isolates 
      affiliated with the GKS98 cluster grew in ecophysiological experiments at maximum 
      salinities of 0.3% to 0.7% (oligosaline), while this group was detected in 
      habitats with salinities up to 6.7% (hypersaline). This observation indicated 
      ecologically significant differences in ecophysiological adaptations among 
      members of this narrow phylogenetic group and suggested ecological significance 
      of microdiversity.
FAU - Wu, Qinglong L
AU  - Wu QL
AD  - Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, East 
      Beijing Road 73, Nanjing 210008, People's Republic of China.
FAU - Zwart, Gabriel
AU  - Zwart G
FAU - Schauer, Michael
AU  - Schauer M
FAU - Kamst-van Agterveld, Miranda P
AU  - Kamst-van Agterveld MP
FAU - Hahn, Martin W
AU  - Hahn MW
LA  - eng
SI  - GENBANK/AM182266
SI  - GENBANK/AM182267
SI  - GENBANK/AM182268
SI  - GENBANK/AM182269
SI  - GENBANK/AM182270
SI  - GENBANK/AM182271
SI  - GENBANK/AM182272
SI  - GENBANK/AM182273
SI  - GENBANK/AM182274
SI  - GENBANK/AM182275
SI  - GENBANK/AM182276
SI  - GENBANK/AM182277
SI  - GENBANK/AM182278
SI  - GENBANK/AM182279
SI  - GENBANK/AM182280
SI  - GENBANK/AM182281
SI  - GENBANK/AM182282
SI  - GENBANK/AM182283
SI  - GENBANK/AM182284
SI  - GENBANK/AM182285
SI  - GENBANK/AM182286
SI  - GENBANK/AM182287
SI  - GENBANK/AM182288
SI  - GENBANK/AM182289
SI  - GENBANK/AM182290
SI  - GENBANK/AM182291
SI  - GENBANK/AM182292
SI  - GENBANK/AM182293
SI  - GENBANK/AM182294
SI  - GENBANK/AM182295
SI  - GENBANK/AM182296
SI  - GENBANK/AM182297
SI  - GENBANK/AM182298
SI  - GENBANK/AM182299
SI  - GENBANK/AM182300
SI  - GENBANK/AM182301
SI  - GENBANK/AM182302
SI  - GENBANK/AM182303
SI  - GENBANK/AM182304
SI  - GENBANK/AM182305
SI  - GENBANK/AM182306
SI  - GENBANK/AM182307
SI  - GENBANK/AM182308
SI  - GENBANK/AM182309
SI  - GENBANK/AM182310
SI  - GENBANK/AM182311
SI  - GENBANK/AM182312
SI  - GENBANK/AM182313
SI  - GENBANK/AM182314
SI  - GENBANK/AM182315
SI  - GENBANK/AM182316
SI  - GENBANK/AM182317
SI  - GENBANK/AM182318
SI  - GENBANK/AM182319
SI  - GENBANK/AM182320
SI  - GENBANK/AM182321
SI  - GENBANK/AM182322
SI  - GENBANK/AM182323
SI  - GENBANK/AM182324
SI  - GENBANK/AM182325
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - *Altitude
MH  - Animals
MH  - China
MH  - Colony Count, Microbial
MH  - DNA, Bacterial/analysis
MH  - DNA, Ribosomal/analysis
MH  - Ecosystem
MH  - Fresh Water/chemistry/*microbiology
MH  - Genes, rRNA
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Plankton/classification/drug effects/genetics/*growth & development
MH  - Proteobacteria/classification/drug effects/genetics/*growth & development
MH  - RNA, Ribosomal, 16S
MH  - Sequence Analysis, DNA
MH  - Sodium Chloride/*pharmacology
PMC - PMC1538701
EDAT- 2006/08/04 09:00
MHDA- 2006/10/06 09:00
PMCR- 2006/12/01
CRDT- 2006/08/04 09:00
PHST- 2006/08/04 09:00 [pubmed]
PHST- 2006/10/06 09:00 [medline]
PHST- 2006/08/04 09:00 [entrez]
PHST- 2006/12/01 00:00 [pmc-release]
AID - 72/8/5478 [pii]
AID - 0767-06 [pii]
AID - 10.1128/AEM.00767-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Aug;72(8):5478-85. doi: 10.1128/AEM.00767-06.