PMID- 16887208
OWN - NLM
STAT- MEDLINE
DCOM- 20061215
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1763
IP  - 9
DP  - 2006 Sep
TI  - Cytoplasmic Ca2+ signals and cellular death by apoptosis in myocardiac H9c2 
      cells.
PG  - 937-47
AB  - The incubation of H9c2 cells with 10 microM thapsigargin (TG) was associated with 
      the appearance of a two-component cytoplasmic Ca2+ peak. Experiments performed in 
      a Ca2+-free medium indicated that both components came from intracellular 
      sources. The first component of the signal corresponded to the discharge of the 
      sarco-endoplasmic reticulum (SER) Ca2+ store. The appearance of the second 
      component was prevented by cell preincubation with cyclosporin A (CsA) and gave 
      rise to a clear and permanent depolarization of the mitochondrial inner membrane. 
      These features were indication of a mitochondrial origin. The observed release of 
      mitochondrial Ca2+ was related with opening of the permeability transition pore 
      (PTP). The two-component cytoplasmic Ca2+ peak, i.e., treatment with 10 microM 
      TG, as compared with the first component alone, i.e., treatment with 3 microM TG, 
      was associated with a faster process of cellular death. In both cases, chromatin 
      fragmentation and condensation at the nuclear periphery were observed. Other 
      prominent apoptotic events such as loss of DNA content and cleavage of 
      poly(ADP-ribose) polymerase (PARP) were also dependent on TG concentration and 
      occurred in different time windows. PTP opening induced by 10 microM TG was 
      responsible for the faster apoptotic death.
FAU - Lax, Antonio
AU  - Lax A
AD  - Departamento de Bioquimica y Biologia Molecular A, Edificio de Veterinaria, 
      Universidad de Murcia, Campus de Espinardo, 30071 Murcia, Spain.
FAU - Soler, Fernando
AU  - Soler F
FAU - Fernandez-Belda, Francisco
AU  - Fernandez-Belda F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060519
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Rhodamines)
RN  - 0 (tetramethylrhodamine methyl ester)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Cytoplasm/*metabolism
MH  - Flow Cytometry
MH  - Immunoblotting
MH  - Microscopy, Fluorescence
MH  - Myocardium/*cytology/metabolism
MH  - Rats
MH  - Rhodamines
MH  - Signal Transduction/*physiology
EDAT- 2006/08/05 09:00
MHDA- 2006/12/16 09:00
CRDT- 2006/08/05 09:00
PHST- 2006/02/15 00:00 [received]
PHST- 2006/05/04 00:00 [revised]
PHST- 2006/05/12 00:00 [accepted]
PHST- 2006/08/05 09:00 [pubmed]
PHST- 2006/12/16 09:00 [medline]
PHST- 2006/08/05 09:00 [entrez]
AID - S0167-4889(06)00113-3 [pii]
AID - 10.1016/j.bbamcr.2006.05.009 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2006 Sep;1763(9):937-47. doi: 10.1016/j.bbamcr.2006.05.009. 
      Epub 2006 May 19.