PMID- 16887642 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20070517 LR - 20060804 IS - 1075-9964 (Print) IS - 1075-9964 (Linking) VI - 4 IP - 4 DP - 1998 Aug TI - Effect of specific inhibitors on the anaerobic reductive dechlorination of 2,4,6-trichlorophenol by a stable methanogenic consortium. PG - 197-203 AB - The transformation of 2,4,6-trichlorophenol (TCP) into 4-chlorophenol (4CP) was studied using a stable methanogenic enrichment culture derived from an anaerobic fixed bed reactor. Using acetate as a growth substrate, different inhibitors of methanogenesis exhibited distinct effects on TCP dechlorination. Whereas reductive dechlorination activity was not affected by 2% ethylene in the gas phase, 25 mM bromoethanesulfonic acid (BESA) had a direct inhibitory effect on this process. The choice of BESA as a specific inhibitor for identifying the subpopulations involved in reductive dechlorination of chloroaromatics is thus questionable. Inhibitors of sulfate reduction such as molybdate (20 mM) and selenate (20 mM) had a direct inhibitory effect on reductive dechlorination independently of the presence of sulfate in the medium supplemented with acetate as growth substrate. Consequently much more care must also be taken with these inhibitors to prove that reductive chlorination is coupled to sulfate reduction. FAU - El Fantroussi, S AU - El Fantroussi S AD - Unit of Bioengineering, Catholic University of Louvain, Place Croix du Sud 2/19, B-1348, Louvain-la-Neuve, Belgium. FAU - Ntibahezwa, E AU - Ntibahezwa E FAU - Thomas, S AU - Thomas S FAU - Naveau, H AU - Naveau H FAU - Agathos, S N AU - Agathos SN LA - eng PT - Journal Article PL - England TA - Anaerobe JT - Anaerobe JID - 9505216 EDAT- 2006/08/05 09:00 MHDA- 2006/08/05 09:01 CRDT- 2006/08/05 09:00 PHST- 1998/06/12 00:00 [received] PHST- 1998/07/29 00:00 [accepted] PHST- 1998/06/12 00:00 [received] PHST- 1998/07/29 00:00 [accepted] PHST- 2006/08/05 09:00 [pubmed] PHST- 2006/08/05 09:01 [medline] PHST- 2006/08/05 09:00 [entrez] AID - S1075-9964(98)90164-0 [pii] AID - 10.1006/anae.1998.0164 [doi] PST - ppublish SO - Anaerobe. 1998 Aug;4(4):197-203. doi: 10.1006/anae.1998.0164.