PMID- 16888087
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20071203
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 80
IP  - 4
DP  - 2006 Oct
TI  - Normal hematopoiesis after conditional targeting of RXRalpha in murine 
      hematopoietic stem/progenitor cells.
PG  - 850-61
AB  - Because of the retinoic acid receptor-alpha (RARalpha) gene's involvement in 
      acute promyelocytic leukemia, the important role of RARs in hematopoiesis is now 
      well established. However, relatively few studies of hematopoiesis have focused 
      on the role of the retinoid X receptors (RXRs), the obligate heterodimeric 
      partners of the RARs. We sought to establish whether conditional targeting of 
      RXRalpha in early hematopoietic progenitors, ideally to the level of the 
      hematopoietic stem cell (HSC), would compromise hematopoiesis. For hematopoietic 
      targeting of RXRalpha, we characterized IFN-inducible MxCre mice for use in 
      studying the role of RXRalpha in hematopoiesis. We established that MxCre 
      executes recombination of loxP-flanked RXRalpha in hematopoietic progenitors 
      immunophenotypically enriched for HSC, leading to widespread and sustained 
      targeting of RXRalpha in hematopoietic cells. However, we found no evidence of 
      hematologic compromise in mice lacking RXRalpha, suggesting that RXRalpha is 
      dispensable for normal murine hematopoiesis. Nonetheless, RXRalpha null bone 
      marrow cells cultured in methylcellulose form colonies more efficiently than bone 
      marrow cells obtained from control mice. This result suggests that although 
      RXRalpha is not required for murine hematopoiesis, there may be hematopoietic 
      signaling pathways that respond selectively to RXRalpha or settings in which 
      combined expression of RXR (alpha, beta, and gamma) is limiting.
FAU - Ricote, Mercedes
AU  - Ricote M
AD  - Department of Cellular and Molecular Medicine, University of California, La 
      Jolla, San Diego, USA. mricote@cnic.es
FAU - Snyder, Cynthia S
AU  - Snyder CS
FAU - Leung, Ho-Yin
AU  - Leung HY
FAU - Chen, Ju
AU  - Chen J
FAU - Chien, Kenneth R
AU  - Chien KR
FAU - Glass, Christopher K
AU  - Glass CK
LA  - eng
GR  - 2 P30 A23100-18/PHS HHS/United States
GR  - CA52599/CA/NCI NIH HHS/United States
GR  - HL67034/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060803
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Retinoid X Receptor beta)
RN  - 0 (Retinoid X Receptor gamma)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Animals
MH  - Gene Expression Profiling
MH  - Hematopoiesis/*immunology
MH  - Hematopoietic Stem Cells/*immunology
MH  - Integrases/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - RNA/genetics
MH  - Retinoid X Receptor alpha/genetics/*immunology
MH  - Retinoid X Receptor beta/genetics/immunology
MH  - Retinoid X Receptor gamma/genetics/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Signal Transduction/immunology
EDAT- 2006/08/05 09:00
MHDA- 2007/09/29 09:00
CRDT- 2006/08/05 09:00
PHST- 2006/08/05 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2006/08/05 09:00 [entrez]
AID - jlb.0206097 [pii]
AID - 10.1189/jlb.0206097 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2006 Oct;80(4):850-61. doi: 10.1189/jlb.0206097. Epub 2006 Aug 3.