PMID- 16888615
OWN - NLM
STAT- MEDLINE
DCOM- 20061107
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 20
IP  - 10
DP  - 2006 Oct
TI  - Novel FISH probes designed to detect IGK-MYC and IGL-MYC rearrangements in B-cell 
      lineage malignancy identify a new breakpoint cluster region designated BVR2.
PG  - 1790-9
AB  - Detection of translocations involving MYC at 8q24.1 in B-cell lineage 
      malignancies (BCL) is important for diagnostic and prognostic purposes. However, 
      routine detection of MYC translocations is often hampered by the wide variation 
      in breakpoint location within the MYC region, particularly when a gene other than 
      IGH, such as IGK or IGL, is involved. To address this issue, we developed and 
      validated four fluorescence in situ hybridization (FISH) probes: two break apart 
      probes to detect IGK and IGL translocations, and two dual-color, dual-fusion FISH 
      (D-FISH) probes to detect IGK-MYC and IGL-MYC. MYC rearrangements (four IGK-MYC, 
      12 IGL-MYC and four unknown partner gene-MYC) were correctly identified in 20 of 
      20 archival BCL specimens known to have MYC rearrangements not involving IGH. 
      Seven specimens, all of which lacked MYC rearrangements using a commercial 
      IGH/MYC D-FISH probe, were found to have 8q24 breakpoints within a cluster region 
      >350-645 kb 3' from MYC, provisionally designated as Burkitt variant 
      rearrangement region 2 (BVR2). FISH is a useful ancillary tool in identifying MYC 
      rearrangements. In light of the discovery of the distally located BVR2 breakpoint 
      cluster region, it is important to use MYC FISH probes that cover a breakpoint 
      region at least 1.0 Mb 3' of MYC.
FAU - Einerson, R R
AU  - Einerson RR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 
      55905, USA.
FAU - Law, M E
AU  - Law ME
FAU - Blair, H E
AU  - Blair HE
FAU - Kurtin, P J
AU  - Kurtin PJ
FAU - McClure, R F
AU  - McClure RF
FAU - Ketterling, R P
AU  - Ketterling RP
FAU - Flynn, H C
AU  - Flynn HC
FAU - Dogan, A
AU  - Dogan A
FAU - Remstein, E D
AU  - Remstein ED
LA  - eng
GR  - CA97274/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060803
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
RN  - 0 (IgK)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - B-Lymphocytes/physiology
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - DNA Probes/genetics
MH  - Gene Rearrangement, B-Lymphocyte/*genetics
MH  - Genes, myc/*genetics
MH  - Genetic Testing/methods
MH  - Humans
MH  - Immunoglobulin Light Chains/genetics
MH  - Immunoglobulins/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, B-Cell/diagnosis/*genetics
MH  - Translocation, Genetic/genetics
EDAT- 2006/08/05 09:00
MHDA- 2006/11/09 09:00
CRDT- 2006/08/05 09:00
PHST- 2006/08/05 09:00 [pubmed]
PHST- 2006/11/09 09:00 [medline]
PHST- 2006/08/05 09:00 [entrez]
AID - 2404340 [pii]
AID - 10.1038/sj.leu.2404340 [doi]
PST - ppublish
SO  - Leukemia. 2006 Oct;20(10):1790-9. doi: 10.1038/sj.leu.2404340. Epub 2006 Aug 3.