PMID- 16889646
OWN - NLM
STAT- MEDLINE
DCOM- 20061121
LR  - 20240109
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 47
IP  - 6
DP  - 2006 Sep
TI  - SERRATE is a novel nuclear regulator in primary microRNA processing in 
      Arabidopsis.
PG  - 841-50
AB  - The Arabidopsis gene SERRATE (SE) controls leaf development, meristem activity, 
      inflorescence architecture and developmental phase transition. It has been 
      suggested that SE, which encodes a C(2)H(2) zinc finger protein, may change gene 
      expression via chromatin modification. Recently, SE has also been shown to 
      regulate specific microRNAs (miRNAs), miR165/166, and thus control shoot meristem 
      function and leaf polarity. However, it remains unclear whether and how SE 
      modulates specific miRNA processing. Here we show that the se mutant exhibits 
      some similar developmental abnormalities as the hyponastic leaves1 (hyl1) mutant. 
      Since HYL1 is a nuclear double-stranded RNA-binding protein acting in the 
      DICER-LIKE1 (DCL1) complex to regulate the first step of primary miRNA transcript 
      (pri-miRNA) processing, we hypothesized that SE could play a previously 
      unrecognized and general role in miRNA processing. Genetic analysis supports that 
      SE and HYL1 act in the same pathway to regulate plant development. Consistently, 
      SE is critical for the accumulation of multiple miRNAs and the trans-acting small 
      interfering RNA (ta-siRNA), but is not required for sense post-transcriptional 
      gene silencing. We further demonstrate that SE is localized in the nucleus and 
      interacts physically with HYL1. Finally, we provide evidence that SE and HYL1 
      probably act with DCL1 in processing pri-miRNAs before HEN1 in miRNA biogenesis. 
      In plants and animals, miRNAs are known to be processed in a stepwise manner from 
      pri-miRNA. Our data strongly suggest that SE plays an important and general role 
      in pri-miRNA processing, and it would be interesting to determine whether animal 
      SE homologues may play similar roles in vivo.
FAU - Yang, Li
AU  - Yang L
AD  - National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant 
      Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese 
      Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
FAU - Liu, Ziqiang
AU  - Liu Z
FAU - Lu, Feng
AU  - Lu F
FAU - Dong, Aiwu
AU  - Dong A
FAU - Huang, Hai
AU  - Huang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060802
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SE protein, Arabidopsis)
RN  - 0 (Serrate-Jagged Proteins)
SB  - IM
MH  - Arabidopsis Proteins
MH  - Calcium-Binding Proteins/metabolism/*physiology
MH  - Cell Nucleus/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism/*physiology
MH  - Membrane Proteins/metabolism/*physiology
MH  - MicroRNAs/*physiology
MH  - RNA Processing, Post-Transcriptional/*physiology
MH  - RNA-Binding Proteins
MH  - Serrate-Jagged Proteins
EDAT- 2006/08/08 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/08 09:00
PHST- 2006/08/08 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/08 09:00 [entrez]
AID - TPJ2835 [pii]
AID - 10.1111/j.1365-313X.2006.02835.x [doi]
PST - ppublish
SO  - Plant J. 2006 Sep;47(6):841-50. doi: 10.1111/j.1365-313X.2006.02835.x. Epub 2006 
      Aug 2.