PMID- 16890264
OWN - NLM
STAT- MEDLINE
DCOM- 20070821
LR  - 20161124
IS  - 1874-9968 (Electronic)
IS  - 0041-624X (Linking)
VI  - 45
IP  - 1-4
DP  - 2006 Dec
TI  - Sonodynamic effects of protoporphyrin IX disodium salt on isolated sarcoma 180 
      cells.
PG  - 56-60
AB  - The cytotoxic effect of PPIX on isolated sarcoma 180 cells induced by ultrasound 
      was investigated. Tumor cells suspended in air-saturated PBS (pH 7.2) were 
      exposed to ultrasound at 2.2 MHz for up to 60s in the presence and absence of 
      protoporphyrin IX disodium salt (PPIX). The viability of cells was determined by 
      a trypan blue exclusion test. The rate of ultrasonically induced cell damage was 
      increased with 40-160 microM PPIX, while no cell damage was observed with 160 
      microM PPIX alone. This enhancement of cell damage with PPIX was inhibited by 
      histidine. The participation of lipid peroxidation products in the cell damage 
      process was also investigated. Scanning electron microscope (SEM) observation of 
      the surface of cells was performed to evaluate the morphological changes induced 
      by ultrasonic irradiation. The results indicate the involvement of a sonochemical 
      mechanism.
FAU - Liu, Quanhong
AU  - Liu Q
AD  - College of Life Science, Shaanxi Normal University, Xi'an, People's Republic of 
      China. lshaof@snnu.edu.cn <lshaof@snnu.edu.cn>
FAU - Wang, Xiaobing
AU  - Wang X
FAU - Wang, Pan
AU  - Wang P
FAU - Qi, Hao
AU  - Qi H
FAU - Zhang, Kun
AU  - Zhang K
FAU - Xiao, Lina
AU  - Xiao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060720
PL  - Netherlands
TA  - Ultrasonics
JT  - Ultrasonics
JID - 0050452
RN  - 0 (Protoporphyrins)
RN  - 0 (Radiation-Sensitizing Agents)
RN  - C2K325S808 (protoporphyrin IX)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Survival/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Lipid Peroxidation/*drug effects
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Protoporphyrins/*administration & dosage
MH  - Radiation Tolerance/*drug effects
MH  - Radiation-Sensitizing Agents/administration & dosage
MH  - Sarcoma/*metabolism/pathology/*therapy
MH  - Treatment Outcome
MH  - Ultrasonic Therapy/*methods
EDAT- 2006/08/08 09:00
MHDA- 2007/08/22 09:00
CRDT- 2006/08/08 09:00
PHST- 2006/08/08 09:00 [pubmed]
PHST- 2007/08/22 09:00 [medline]
PHST- 2006/08/08 09:00 [entrez]
AID - S0041-624X(06)00308-8 [pii]
AID - 10.1016/j.ultras.2006.06.063 [doi]
PST - ppublish
SO  - Ultrasonics. 2006 Dec;45(1-4):56-60. doi: 10.1016/j.ultras.2006.06.063. Epub 2006 
      Jul 20.