PMID- 16891764
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20190911
IS  - 1347-8613 (Print)
IS  - 1347-8613 (Linking)
VI  - 101
IP  - 4
DP  - 2006 Aug
TI  - Protein kinase C beta inhibitor LY333531 attenuates intercellular adhesion 
      molecule-1 and monocyte chemotactic protein-1 expression in the kidney in 
      diabetic rats.
PG  - 335-43
AB  - In vitro studies have shown that activation of protein kinase C (PKC) is a key 
      mediator of intercellular adhesion molecule-1 (ICAM-1) and monocyte 
      chemoattractant protein-1 (MCP-1) in a range of cell types and in response to 
      high glucose, however, its role in the in vivo setting has not been clearly 
      delineated. Streptozotocin-induced diabetic rats were treated with the PKC-beta 
      isoform inhibitor LY333531 for 8 weeks. LY333531 treatment significantly 
      attenuated increased urinary albumin excretion rate and glomerular volume and 
      tubulointerstitial injury index as well as elevated PKC activity and PKC-beta 
      protein expression in the kidney. Level of malondialdehyde was markedly higher 
      and antioxidant enzyme activity such as superoxide diamutase and catalase as well 
      as glutathione peroxidase were significantly lower in the kidney from diabetic 
      rats than that of the control group. LY333531 administration could remit these 
      changes. Increased macrophages recruitment as well as ICAM-1 and MCP-1 protein 
      expression in the kidney were significantly inhibited by LY333531 in diabetic 
      rats. It is concluded that mechanism of renoprotection of LY333531 may be 
      correlated, at least partly, with suppression of increased macrophages 
      recruitment and overexpression of ICAM-1 and MCP-1 in diabetic rats.
FAU - Wu, Yonggui
AU  - Wu Y
AD  - Department of Nephropathy, the First Affiliated Hospital of AnHui Medical 
      University, Hefei, China. wygxll@ah163.com
FAU - Wu, Guozhong
AU  - Wu G
FAU - Qi, Xiangming
AU  - Qi X
FAU - Lin, Hui
AU  - Lin H
FAU - Qian, Hao
AU  - Qian H
FAU - Shen, Jijia
AU  - Shen J
FAU - Lin, Shantan
AU  - Lin S
LA  - eng
PT  - Journal Article
DEP - 20060805
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Blood Glucose)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Indoles)
RN  - 0 (LY 338522)
RN  - 0 (Maleimides)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C beta)
SB  - IM
MH  - Albuminuria/prevention & control
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Blotting, Western
MH  - Body Weight/drug effects
MH  - Chemokine CCL2/*biosynthesis
MH  - Diabetes Mellitus, Experimental/complications/drug therapy/*metabolism
MH  - Immunohistochemistry
MH  - Indoles/*pharmacology
MH  - Intercellular Adhesion Molecule-1/*biosynthesis
MH  - Kidney/*drug effects/metabolism/pathology
MH  - Kidney Glomerulus/drug effects/metabolism/pathology
MH  - Macrophages/drug effects/immunology/metabolism
MH  - Maleimides/*pharmacology
MH  - Malondialdehyde/antagonists & inhibitors/metabolism
MH  - Nephritis, Interstitial/complications/drug therapy
MH  - Organ Size/drug effects
MH  - Oxidative Stress/drug effects
MH  - Peroxidases/antagonists & inhibitors/metabolism
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Protein Kinase C beta
MH  - Rats
MH  - Rats, Wistar
EDAT- 2006/08/08 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/08/08 09:00
PHST- 2006/08/08 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/08/08 09:00 [entrez]
AID - JST.JSTAGE/jphs/FP0050896 [pii]
AID - 10.1254/jphs.fp0050896 [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2006 Aug;101(4):335-43. doi: 10.1254/jphs.fp0050896. Epub 2006 
      Aug 5.