PMID- 16892139
OWN - NLM
STAT- MEDLINE
DCOM- 20080527
LR  - 20171116
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 38
IP  - 4
DP  - 2006 Aug 18
TI  - [Induction of skin allograft tolerance in mice by using anti-alphabeta T cell 
      receptor and anti-CD80 monoclonal antibodies combined with bone marrow 
      transfusion].
PG  - 365-9
AB  - OBJECTIVE: To explore the role of anti-alphabeta T cell receptor (TCR) and 
      anti-CD80 monoclonal antibodies (mAbs) combined with donor bone marrow cells 
      (BMCs) infusion in the induction of murine skin allografts tolerance. METHODS: On 
      day 0, 2 x 10(8) BMCs of BALB/c mice were injected into recipient C57BL/6 mice 
      via the tail vein, meanwhile, an intraperitoneal injection of TCRalphabeta mAb 
      (500 microg) was given. On day 2, CD80 mAb was administered intraperitoneally. 
      Skin grafting was performed on day 6. Delayed type hypersensitivity (DTH), mixed 
      lymphocyte reaction (MLR), IL-2 reverse assay of MLR, adoptive transfer assay and 
      chimerism detection were performed at different time points and tolerance 
      mechanisms were investigated. RESULTS: The mean survival time (MST) of BALB/c 
      skin allografts in C57BL/6 recipients that were treated by anti-TCRalphabeta and 
      anti-CD80 mAbs combined with donor BMCs infusion was 70 days. DTH and MLR assay 
      indicated that the tolerant mice displayed significant hyporesponsiveness. The 
      result of IL-2 reverse test showed that clone anergy was probably involved in the 
      formation of tolerance in the tolerant C57BL/6 mice. In vivo and in vitro 
      adoptive transfer assay, suppressive activity in the spleens of tolerant C57BL/6 
      mice was observed. Chimerism existed in both the thymus and spleen of the 
      tolerant C57BL/6 mice. The chimerism level gradually declined with time. 
      CONCLUSION: Treatment of anti-TCRalphabeta and anti-CD80 mAbs combined with donor 
      BMCs infusion can successfully induce a long-term tolerance in BALB/c mice to 
      C57BL/6 skin graft. Multiple mechanisms, including clone anergy, suppressor cells 
      and chimerism are involved in the tolerance.
FAU - Hao, Jie
AU  - Hao J
AD  - Department of Immunology, School of Basic Medical Sciences, Peking University, 
      Beijing 100083, China.
FAU - Liu, Jia-wang
AU  - Liu JW
FAU - Gao, Xiang
AU  - Gao X
FAU - Yuan, Guo-hong
AU  - Yuan GH
FAU - Xie, Shu-sheng
AU  - Xie SS
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD8 Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*administration & dosage/immunology
MH  - *Bone Marrow Transplantation
MH  - CD8 Antigens/*immunology
MH  - Clonal Anergy/immunology
MH  - Female
MH  - Immune Tolerance/drug effects/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Receptors, Antigen, T-Cell, alpha-beta/*immunology
MH  - Skin Transplantation/*immunology
EDAT- 2006/08/08 09:00
MHDA- 2008/05/28 09:00
CRDT- 2006/08/08 09:00
PHST- 2006/08/08 09:00 [pubmed]
PHST- 2008/05/28 09:00 [medline]
PHST- 2006/08/08 09:00 [entrez]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Aug 18;38(4):365-9.