PMID- 1689215
OWN - NLM
STAT- MEDLINE
DCOM- 19900323
LR  - 20191021
IS  - 0008-8730 (Print)
IS  - 0008-8730 (Linking)
VI  - 23
IP  - 1
DP  - 1990 Jan
TI  - Caffeine increases sensitivity of DNA to denaturation in chromatin of L1210 
      cells.
PG  - 31-9
AB  - Exposure of exponentially growing L1210 cells to 5 mM and higher concentrations 
      of caffeine perturbs their progression through the cell cycle and results in 
      increased sensitivity of DNA in situ to denaturation. The latter is detected by 
      the increased metachromatic stainability of DNA with acridine orange (AO) and 
      sensitivity to S1 nuclease, measured by flow cytometry. Decreased DNA stability 
      is generally characteristic of chromatin condensation and in untreated cells is 
      observed in mitosis or quiescence (G0). The caffeine-induced decrease in DNA 
      stability affects the interphase cells regardless of their position in the cycle 
      and the changes are stochastic, concentration- and time-dependent. Populations of 
      cells responding to caffeine are very heterogenous with respect to the degree of 
      destabilization of DNA; sensitivity of DNA to denaturation of the maximally 
      affected cells is similar to that of untreated cells in mitosis. The present 
      method allows one to quantitatively express effects of caffeine on nuclear 
      chromatin in individual cells of large cell populations and may be employed in 
      studies correlating chromatin changes induced by this agent with its effects in 
      modulation of cell sensitivity to radiation or antitumour drugs.
FAU - Kunicka, J E
AU  - Kunicka JE
AD  - Memorial Sloan-Kettering Cancer Center, New York, N.Y. 10021.
FAU - Myc, A
AU  - Myc A
FAU - Melamed, M R
AU  - Melamed MR
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
LA  - eng
GR  - CA 23296/CA/NCI NIH HHS/United States
GR  - CA 28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Tissue Kinet
JT  - Cell and tissue kinetics
JID - 0174107
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 3G6A5W338E (Caffeine)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Caffeine/*pharmacology
MH  - Chromatin/*drug effects
MH  - DNA, Neoplasm/analysis/*drug effects
MH  - Flow Cytometry
MH  - Interphase
MH  - Leukemia L1210/metabolism/*pathology
MH  - Mice
MH  - Nucleic Acid Denaturation/*drug effects
MH  - Staining and Labeling
MH  - Tumor Cells, Cultured
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1111/j.1365-2184.1990.tb01107.x [doi]
PST - ppublish
SO  - Cell Tissue Kinet. 1990 Jan;23(1):31-9. doi: 10.1111/j.1365-2184.1990.tb01107.x.