PMID- 16893385
OWN - NLM
STAT- MEDLINE
DCOM- 20060914
LR  - 20221207
IS  - 1744-3121 (Print)
IS  - 1744-3121 (Linking)
VI  - 33
IP  - 4
DP  - 2006 Aug
TI  - MICA-STR, HLA-B haplotypic diversity and linkage disequilibrium in the Hunan Han 
      population of southern China.
PG  - 241-5
AB  - Major histocompatibility complex (MHC) class I chain-related gene A (MICA) is 
      located 46 kb centromeric to HLA-B and encodes a stress-inducible protein. MICA 
      allelic variation is thought to be associated with disease susceptibility and 
      immune response to transplants. This study was aimed to investigate the 
      haplotypic diversity and linkage disequilibrium between human leukocyte antigen 
      (HLA)-B and (GCT)(n) short tandem repeat in exon 5 of MICA gene (MICA-STR) in a 
      southern Chinese Han population. Fifty-eight randomly selected nuclear families 
      with 183 members including 85 unrelated parental samples were collected in Hunan 
      province, southern China. HLA-B generic typing was performed by polymerase chain 
      reaction-sequence-specific priming (PCR-SSP), and samples showing novel 
      HLA-B-MICA-STR linkage were further typed for HLA-B allelic variation by 
      high-resolution PCR-SSP. MICA-STR allelic variation and MICA gene deletion 
      (MICA*Del) were detected by fluorescent PCR-size sequencing and PCR-SSP. 
      Haplotype was determined through family segregation analysis. Statistical 
      analysis was applied to the data of the 85 unrelated parental samples. Nineteen 
      HLA-B specificities and seven MICA-STR allelic variants were observed in 85 
      unrelated parental samples, the most predominant of which were HLA-B*46, -B60, 
      -B*13, and -B*15, and MICA*A5, MICA*A5.1 and MICA*A4, respectively. Genotype 
      distributions of HLA-B, MICA-STR loci were consistent with Hardy-Weinberg 
      proportions. The HLA-B-MICA-STR haplotypic phases of all 85 unrelated parental 
      samples were unambiguously assigned, which contained 30 kinds of HLA-B, MICA-STR 
      haplotypic combinations, nine of them have not been reported in the literature. 
      Significant positive linkage disequilibria between certain HLA-B and MICA-STR 
      alleles, including HLA-B*13 and MICA*A4, HLA-B*38 and MICA*A9, HLA-B*58 and 
      MICA*A9, HLA-B*46 and MICA*A5, HLA-B*51 and MICA*A6, HLA-B*52 and MICA*A6, and 
      HLA-B60 and MICA*A5.1, were observed. HLA-B*48 was linked to MICA*A5, MICA*A5.1 
      and MICA*Del. HLA-B*5801-MICA*A10 linkage was found in a family. Our data 
      indicated a high degree of haplotypic diversity and strong linkage disequilibrium 
      between MICA-STR and HLA-B in a southern Chinese Han population, the data will 
      inform future studies on anthropology, donor-recipient HLA matching in clinical 
      transplantation and HLA-linked disease association.
FAU - Tian, W
AU  - Tian W
AD  - Department of Immunology, Central South University Xiang-Ya School of 
      Medicine,Changsha, Hunan, China. tianwei3@yahoo.com
FAU - Li, L-X
AU  - Li LX
FAU - Wang, F
AU  - Wang F
FAU - Luo, Q-Z
AU  - Luo QZ
FAU - Yan, M-Y
AU  - Yan MY
FAU - Yu, P
AU  - Yu P
FAU - Guo, S-S
AU  - Guo SS
FAU - Cao, Y
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Immunogenet
JT  - International journal of immunogenetics
JID - 101232337
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (MHC class I-related chain A)
SB  - IM
MH  - Asian People/*genetics
MH  - China
MH  - Ethnicity/genetics
MH  - Exons
MH  - Family
MH  - HLA-B Antigens/*genetics
MH  - Haplotypes
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - *Linkage Disequilibrium
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Tandem Repeat Sequences/*genetics
EDAT- 2006/08/09 09:00
MHDA- 2006/09/15 09:00
CRDT- 2006/08/09 09:00
PHST- 2006/08/09 09:00 [pubmed]
PHST- 2006/09/15 09:00 [medline]
PHST- 2006/08/09 09:00 [entrez]
AID - EJI605 [pii]
AID - 10.1111/j.1744-313X.2006.00605.x [doi]
PST - ppublish
SO  - Int J Immunogenet. 2006 Aug;33(4):241-5. doi: 10.1111/j.1744-313X.2006.00605.x.