PMID- 16897489
OWN - NLM
STAT- MEDLINE
DCOM- 20061016
LR  - 20181113
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 61
IP  - 4-5
DP  - 2006 Jul
TI  - Regulation of the kinase activity of the MIK GCK-like MAP4K by alternative 
      splicing.
PG  - 747-56
AB  - Alternative splicing of introns is essential to ensure the complexity of 
      mammalian genome functions. In particular, the generation of a high number of 
      different isoforms by alternative splicing is an important characteristic of 
      genes coding for signalling proteins such as mitogen activated protein kinases 
      (MAPKs). This is thought to allow these proteins to transduce multiple stimuli in 
      a highly regulated manner. Plant genes are also subjected to alternative 
      splicing. Nevertheless, clear examples of the functional consequences of this 
      phenomenon are still scarce in plants. MIK is a maize gene coding for a GCK-like 
      MAP4K that can be activated by interaction with maize atypical receptor kinase 
      (MARK), an atypical receptor kinase. Here we show that MIK is subjected to 
      alternative splicing. Expression of MIK leads to, at least, 4 different mature 
      mRNAs that accumulate with particular expression profiles during maize 
      development. Our results show that the polypeptides encoded by the different MIK 
      mRNAs display different kinase activity and are differentially activated by 
      interaction with the MARK receptor. Two MIK isoforms display constitutive kinase 
      activity, one isoform is inactive but can be activated by MARK, and the fourth 
      MIK isoform is inactive and cannot be activated by MARK. Our results constitute a 
      clear example of the biochemical consequences of alternative splicing in plants. 
      The selective conservation during evolution of the intron-exon structure of the 
      region coding for the regulator domain of MIK, as well as the maintenance in 
      maize, rice and Arabidopsis of the alternative splicing of some of these introns, 
      are strong indications of its functional importance.
FAU - Castells, Enric
AU  - Castells E
AD  - Departament de Genetica Molecular, Laboratori de Genetica Molecular Vegetal 
      CSIC-IRTA, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Jordi Girona 
      18, 08034 Barcelona, Spain.
FAU - Puigdomenech, Pere
AU  - Puigdomenech P
FAU - Casacuberta, Josep M
AU  - Casacuberta JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - *Alternative Splicing
MH  - Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Plant
MH  - Mitogen-Activated Protein Kinase Kinases/*genetics/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Zea mays/*enzymology/genetics
EDAT- 2006/08/10 09:00
MHDA- 2006/10/17 09:00
CRDT- 2006/08/10 09:00
PHST- 2005/06/17 00:00 [received]
PHST- 2006/03/15 00:00 [accepted]
PHST- 2006/08/10 09:00 [pubmed]
PHST- 2006/10/17 09:00 [medline]
PHST- 2006/08/10 09:00 [entrez]
AID - 10.1007/s11103-006-0046-3 [doi]
PST - ppublish
SO  - Plant Mol Biol. 2006 Jul;61(4-5):747-56. doi: 10.1007/s11103-006-0046-3.