PMID- 16898154
OWN - NLM
STAT- MEDLINE
DCOM- 20061205
LR  - 20190917
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 54
IP  - 1
DP  - 2006
TI  - Microbial community development in a laboratory-scale nitrifying activated sludge 
      system with input from a side-stream bioreactor treating digester supernatant.
PG  - 209-16
AB  - Three laboratory-scale activated sludge treatment trains were operated to 
      investigate the effect on biodiversity in plug flow (PFR) main-stream sewage 
      treatment from input of biomass from side-stream reactors treating anaerobic 
      digester supernatant. One train had a completely mixed (CSTR) side-stream 
      reactor, one a PFR side-stream reactor, and the third train was a control that 
      did not receive input from a side-stream reactor. Restriction endonucleases were 
      used to digest polymerase chain reaction-amplified ammonia monooxygenase subunit 
      A (amoA) genes in monthly samples from each reactor. Restriction fragment banding 
      patterns from polyacrylimide gel electrophoresis indicated that the structure of 
      the ammonia oxidizing bacteria (AOB) populations in all five reactors stabilized 
      by the fourth month of operation and then did not vary subsequently. Furthermore, 
      a dendrogram generated using the Jaccard distance showed that the AOB in each 
      side-stream reactor was most similar to the main-stream reactor in the same train 
      indicating that the AOB population in the side-stream reactor exerts a strong 
      influence on the population in the main-stream reactor. Sequencing results 
      indicated that Nitrosomonas europea, an r-strategist, was the dominant AOB in the 
      PFR side-stream reactor, while Nitrosomonas europea and Nitrosomonas marina, a 
      marine bacterium, were strongly represented in the CSTR side-stream reactor.
FAU - Smith, R C
AU  - Smith RC
AD  - Department of Civil and Environmental Engineering, University of Cincinnati, 
      Cincinnati, OH 45221, USA. smr0@email.uc.edu
FAU - Oerther, D B
AU  - Oerther DB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA Primers)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Base Sequence
MH  - *Bioreactors
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Nitrites/*metabolism
MH  - Nitrosomonas/classification/*metabolism
MH  - Oxidoreductases/genetics/metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Bacterial/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/classification/genetics
MH  - Sewage/*microbiology
EDAT- 2006/08/11 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/11 09:00
PHST- 2006/08/11 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/11 09:00 [entrez]
AID - 10.2166/wst.2006.389 [doi]
PST - ppublish
SO  - Water Sci Technol. 2006;54(1):209-16. doi: 10.2166/wst.2006.389.