PMID- 16898155
OWN - NLM
STAT- MEDLINE
DCOM- 20061205
LR  - 20190917
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 54
IP  - 1
DP  - 2006
TI  - Towards exposure of elusive metabolic mixed-culture processes: the application of 
      metaproteomic analyses to activated sludge.
PG  - 217-26
AB  - Protein expression is a direct reflection of specific microbial activities in any 
      ecosystem. In order to assess protein expression in mixed microbial communities, 
      the feasibility of applying proteomic techniques to activated sludge samples has 
      recently been demonstrated. We report the application of metaproteomics to two 
      activated sludges from a laboratory-scale sequencing batch reactor with 
      dissimilar phosphorus removal performances. Fluorescence in situ hybridization 
      (FISH) revealed that the sludge with good enhanced biological phosphorus removal 
      performance (EBPR) was dominated by Betaproteobacteria (65% of EUBMIX binding 
      cells) and gave positive signals for the Rhodocyclus-type PAO specific probe 
      (59%). The non-EBPR sludge was dominated by tetrad-forming Alphaproteobacteria 
      (75%). With regard to the proteomic investigation, 630 individual protein spots 
      were matched across the replicate groups of the anaerobic and aerobic phases of 
      the EBPR sludge with 9.4% of all spots being statistically different between the 
      two phases. The non-EBPR metaproteomic maps exhibited 590 matched spots with 
      14.7% statistical differences between the two phases. Overall, the non-EBPR 
      sludge expressed around 30% more significant differences than the EBPR sludge. 
      The comparison of protein expression in the two sludges showed that their 
      metaproteomes were substantially different and this was reflected in their 
      microbial community structures and metabolic transformations.
FAU - Wilmes, P
AU  - Wilmes P
AD  - School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 
      7TJ, UK. P.Wilmes@uea.ac.uk
FAU - Bond, P L
AU  - Bond PL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA Primers)
RN  - 0 (Proteome)
RN  - 0 (Sewage)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Ecosystem
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - In Situ Hybridization, Fluorescence
MH  - Proteobacteria/isolation & purification/metabolism
MH  - *Proteome
MH  - Rhodocyclaceae/isolation & purification/metabolism
MH  - Sewage/*microbiology
EDAT- 2006/08/11 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/11 09:00
PHST- 2006/08/11 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/11 09:00 [entrez]
AID - 10.2166/wst.2006.390 [doi]
PST - ppublish
SO  - Water Sci Technol. 2006;54(1):217-26. doi: 10.2166/wst.2006.390.