PMID- 16899765
OWN - NLM
STAT- MEDLINE
DCOM- 20070125
LR  - 20161124
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 55
IP  - 1
DP  - 2007 Jan
TI  - Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus 
      monkey brains.
PG  - 1-19
AB  - Immunohistochemical distribution and cellular localization of neurotrophins was 
      investigated in adult monkey brains using antisera against nerve growth factor 
      (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and 
      neurotrophin-4 (NT-4). Western blot analysis showed that each antibody 
      specifically recognized appropriate bands of approximately 14.7 kDa, 14.2 kDa, 
      13.6 kDa, and 14.5 kDa, for NGF, BDNF, NT-3, and NT-4, respectively. These 
      positions coincided with the molecular masses of the neurotrophins studied. 
      Furthermore, sections exposed to primary antiserum preadsorbed with full-length 
      NGF, BDNF, NT-3, and NT-4 exhibited no detectable immunoreactivity, demonstrating 
      specificities of the antibodies against the tissues prepared from rhesus monkeys. 
      The study provided a systematic report on the distribution of NGF, BDNF, NT-3, 
      and NT-4 in the monkey brain. Varying intensity of immunostaining was observed in 
      the somata and processes of a wide variety of neurons and glial cells in the 
      cerebrum, cerebellum, hippocampus, and other regions of the brain. Neurons in 
      some regions such as the cerebral cortex and the hippocampus, which stained for 
      neurotrophins, also expressed neurotrophic factor mRNA. In some other brain 
      regions, there was discrepancy of protein distribution and mRNA expression 
      reported previously, indicating a retrograde or anterograde action mode of 
      neurotrophins. Results of this study provide a morphological basis for the 
      elucidation of the roles of NGF, BDNF, NT-3, and NT-4 in adult primate brains.
FAU - Zhang, Hong-Tian
AU  - Zhang HT
AD  - Institute for Research on Neuroscience, Kunming Medical College, Kunming, China.
FAU - Li, Li-Yan
AU  - Li LY
FAU - Zou, Xiao-Li
AU  - Zou XL
FAU - Song, Xin-Bo
AU  - Song XB
FAU - Hu, Yan-Li
AU  - Hu YL
FAU - Feng, Zhong-Tang
AU  - Feng ZT
FAU - Wang, Tim Ting-Hua
AU  - Wang TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060809
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Brain/anatomy & histology/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Immunohistochemistry
MH  - Macaca mulatta
MH  - Nerve Growth Factor/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Neuroglia/metabolism
MH  - Neurons/metabolism
MH  - Neurotrophin 3/*metabolism
EDAT- 2006/08/11 09:00
MHDA- 2007/01/26 09:00
CRDT- 2006/08/11 09:00
PHST- 2006/08/11 09:00 [pubmed]
PHST- 2007/01/26 09:00 [medline]
PHST- 2006/08/11 09:00 [entrez]
AID - jhc.6A6952.2006 [pii]
AID - 10.1369/jhc.6A6952.2006 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2007 Jan;55(1):1-19. doi: 10.1369/jhc.6A6952.2006. Epub 
      2006 Aug 9.