PMID- 16899813
OWN - NLM
STAT- MEDLINE
DCOM- 20070321
LR  - 20220409
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 28
IP  - 1
DP  - 2007 Jan-Feb
TI  - Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm 
      chromatin dispersion (SCD) test and FISH analysis.
PG  - 38-49
AB  - Previous studies suggest that sperm DNA fragmentation may be associated with 
      aneuploidy. However, currently available tests have not made it possible to 
      simultaneously perform DNA fragmentation and chromosomal analyses on the same 
      sperm cell. The recently introduced sperm chromatin dispersion (SCD) test allows 
      users to determine this relationship. Semen samples from 16 males, including 4 
      fertile donors, 7 normozoospermic, 3 teratozoospermic, 1 asthenozoospermic, and 1 
      oligoasthenoteratozoospermic, were processed for DNA fragmentation analysis by 
      the SCD test using the Halosperm kit. Three-color fluorescence in situ 
      hybridization (FISH) was performed on SCD-processed slides to determine 
      aneuploidy for chromosomes X, Y, and 18. Spermatozoa with DNA fragmentation 
      showed a 4.4 +/- 1.9-fold increase in diploidy rate and a 5.9 +/- 3.5-fold 
      increase in disomy rate compared to spermatozoa without DNA fragmentation. The 
      overall aneuploidy rate was 4.6 +/- 2.0-fold higher in sperm with fragmented DNA 
      (Wilcoxon rank test: P < .001 in the 3 comparisons). A higher frequency of DNA 
      fragmentation was found in sperm cells containing sex chromosome aneuploidies 
      originated in both first and second meiotic divisions. The observed increase in 
      aneuploidy rate in sperm with fragmented DNA may suggest that the occurrence of 
      aneuploidy during sperm maturation may lead to sperm DNA fragmentation as part of 
      a genomic screening mechanism developed to genetically inactivate sperm with a 
      defective genomic makeup.
FAU - Muriel, Lourdes
AU  - Muriel L
AD  - Seccion de Genetica y Unidad de Investigacion, Complejo Hospitalario 
      Universitario Juan Canalejo, As Xubias, 84, 15006-A Coruna, Spain.
FAU - Goyanes, Vicente
AU  - Goyanes V
FAU - Segrelles, Enrique
AU  - Segrelles E
FAU - Gosalvez, Jaime
AU  - Gosalvez J
FAU - Alvarez, Juan G
AU  - Alvarez JG
FAU - Fernandez, Jose Luis
AU  - Fernandez JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060809
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Chromatin)
SB  - IM
MH  - *Aneuploidy
MH  - Chromatin
MH  - Cytological Techniques
MH  - *DNA Fragmentation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/genetics
MH  - Male
MH  - Spermatozoa/*pathology
EDAT- 2006/08/11 09:00
MHDA- 2007/03/22 09:00
CRDT- 2006/08/11 09:00
PHST- 2006/08/11 09:00 [pubmed]
PHST- 2007/03/22 09:00 [medline]
PHST- 2006/08/11 09:00 [entrez]
AID - jandrol.106.000067 [pii]
AID - 10.2164/jandrol.106.000067 [doi]
PST - ppublish
SO  - J Androl. 2007 Jan-Feb;28(1):38-49. doi: 10.2164/jandrol.106.000067. Epub 2006 
      Aug 9.