PMID- 16900381
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 386
IP  - 5
DP  - 2006 Nov
TI  - Development of a bienzyme system for the electrochemical determination of nitrate 
      in ambient air.
PG  - 1567-70
AB  - This work reports the development of a bienzyme system consisting of salicylate 
      hydroxylase (SHL) and nitrate reductase (NaR) for the electrochemical 
      determination of nitrate. This method measures the concentration of nitrate 
      directly under ambient air without suffering from oxygen interferences. The 
      determination is based on the detection of NADH consumption, and the principle is 
      as follows: NADH initiates the irreversible decarboxylation and hydroxylation of 
      salicylate by SHL in the presence of oxygen to produce catechol, which results in 
      a detectable signal due to its oxidation at the working electrode; the second 
      enzyme, NaR, in the presence of nitrate, reduced the availability of NADH, and 
      consequently, the current difference after the injection of nitrate is 
      proportional to its concentration. This method shows high performance 
      characteristics for nitrate determination with a broad detection range between 10 
      microM and 1,000 microM, a short measuring time of around 5 min, and a simple 
      operation without sample pretreatment by inert gas purge or oxygen scavenger.
FAU - Cui, Yue
AU  - Cui Y
AD  - Department of Chemical Engineering and Department of Chemistry, Hong Kong 
      University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, 
      People's Republic of China. kecuiyue@ust.hk
FAU - Barford, John P
AU  - Barford JP
FAU - Renneberg, Reinhard
AU  - Renneberg R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060810
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Air Pollutants)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Nitrates)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.13.1 (salicylate 1-monooxygenase)
RN  - EC 1.7.99.4 (Nitrate Reductase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Air Pollutants/*chemistry
MH  - Biosensing Techniques/*methods
MH  - Electrochemistry
MH  - Electrodes
MH  - Enzymes, Immobilized/*chemistry
MH  - Mixed Function Oxygenases/*chemistry
MH  - Nitrate Reductase/*chemistry
MH  - Nitrates/*analysis
MH  - Oxygen/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2006/08/11 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/08/11 09:00
PHST- 2006/05/14 00:00 [received]
PHST- 2006/07/06 00:00 [accepted]
PHST- 2006/06/27 00:00 [revised]
PHST- 2006/08/11 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/08/11 09:00 [entrez]
AID - 10.1007/s00216-006-0673-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2006 Nov;386(5):1567-70. doi: 10.1007/s00216-006-0673-1. Epub 
      2006 Aug 10.