PMID- 16904077
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 348
IP  - 3
DP  - 2006 Sep 29
TI  - Allele-specific MMP-3 transcription under in vivo conditions.
PG  - 1150-6
AB  - A common matrix metalloproteinases-3 (MMP-3) -1612 5A/6A promoter polymorphism is 
      associated with risk for cardiovascular disease, rheumatoid arthritis, and other 
      diseases. Here we used the haplotype chromatin immunoprecipitation method to 
      study allele-specific MMP-3 expression under in vivo conditions in heterozygous 
      THP-1 cells. Pyrosequencing was used to analyse the ratio of 5A-allele to 
      6A-allele after chromatin immunoprecipitation using an antibody against 
      phosphorylated active RNA polymerase II. There was no allele-specific difference 
      in transcriptional activity during basal conditions, i.e., in unstimulated 
      monocytic THP-1 cells. However, after stimulation of MMP-3 expression by monocyte 
      differentiation or incubation with IL-1beta, the haplotype containing the 
      5A-allele was associated with higher transcriptional activity compared with the 
      6A-containing haplotype. Electromobility shift assay demonstrated increased 
      binding of nuclear proteins to the 5A-allele after monocyte differentiation. In 
      conclusion, the common MMP-3 5A/6A promoter polymorphism appears to be functional 
      only during specific environmental conditions involving inflammation.
FAU - Zhu, Chaoyong
AU  - Zhu C
AD  - Atherosclerosis Research Unit, King Gustav V Research Institute, Department of 
      Medicine, Karolinska Institute, Stockholm, Sweden.
FAU - Odeberg, Jacob
AU  - Odeberg J
FAU - Hamsten, Anders
AU  - Hamsten A
FAU - Eriksson, Per
AU  - Eriksson P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060804
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - *Alleles
MH  - Cell Line, Tumor
MH  - Humans
MH  - Matrix Metalloproteinase 3/biosynthesis/*genetics
MH  - Monocytes/metabolism
MH  - Polymorphism, Genetic
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - *Transcription, Genetic
EDAT- 2006/08/15 09:00
MHDA- 2006/10/25 09:00
CRDT- 2006/08/15 09:00
PHST- 2006/07/18 00:00 [received]
PHST- 2006/07/28 00:00 [accepted]
PHST- 2006/08/15 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/08/15 09:00 [entrez]
AID - S0006-291X(06)01752-9 [pii]
AID - 10.1016/j.bbrc.2006.07.174 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Sep 29;348(3):1150-6. doi: 
      10.1016/j.bbrc.2006.07.174. Epub 2006 Aug 4.