PMID- 1690411
OWN - NLM
STAT- MEDLINE
DCOM- 19900423
LR  - 20131121
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 186
IP  - 1
DP  - 1990 Feb
TI  - Electron microscopic localization of acridine orange chromatin interaction 
      products in rat pheochromocytoma PC12 cells.
PG  - 102-9
AB  - The purpose of the present study is to examine the distribution pattern of 
      acridine orange (AO) binding to DNA in rat adrenal pheochromocytoma PC12 cells 
      that respond to nerve growth factor (NGF). PC12 cells were incubated in a medium 
      containing 100 ng/ml of NGF for 1-14 days for AO ultracytochemistry. Electron 
      microscopic studies revealed that AO binds to DNA exclusively within the 
      euchromatin portion of the cell nucleus. About 35% of the untreated PC12 cells 
      showed characteristic electron-dense interaction products within the nuclei. The 
      average numbers of AO chromatin interaction products per cell nucleus and per 
      micron 2 nuclear area were 45 and 1.6, respectively. In the presence of NGF, cell 
      proliferation was suppressed. The cells gradually extend neurite-like cell 
      processes. Mean 3H-labeling index was 40.0 +/- 2.2% in untreated cells and 13.0 
      +/- 2.3% in PC12 cells with NGF for 6 days after incubation for 30 min. With 
      increasing the cellular differentiation percentages of AO positive cells and 
      average numbers of AO chromatin interaction products per cell nucleus and per 
      micron 2 nuclear area showed a progressive decrease. The results of the present 
      and previous studies suggest that AO chromatin interaction products may be 
      indicative of cell proliferation and differentiation.
FAU - Hara, H
AU  - Hara H
AD  - Department of Pathology, Kochi Medical School Hospital Nankoku, Japan.
FAU - Moriki, T
AU  - Moriki T
FAU - Togo, M
AU  - Togo M
FAU - Ogawa, M
AU  - Ogawa M
FAU - Yamane, T
AU  - Yamane T
FAU - Lehmann, R
AU  - Lehmann R
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Nerve Growth Factors)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adrenal Gland Neoplasms/*ultrastructure
MH  - Animals
MH  - Autoradiography
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Chromatin/*analysis
MH  - DNA, Neoplasm/*analysis
MH  - Microscopy, Electron
MH  - Nerve Growth Factors/pharmacology
MH  - Pheochromocytoma/*ultrastructure
MH  - Rats
MH  - *Staining and Labeling
MH  - Tumor Cells, Cultured/drug effects/ultrastructure
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - S0344-0338(11)81017-4 [pii]
AID - 10.1016/S0344-0338(11)81017-4 [doi]
PST - ppublish
SO  - Pathol Res Pract. 1990 Feb;186(1):102-9. doi: 10.1016/S0344-0338(11)81017-4.