PMID- 1690412
OWN - NLM
STAT- MEDLINE
DCOM- 19900423
LR  - 20131121
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 186
IP  - 1
DP  - 1990 Feb
TI  - Distribution pattern of acridine orange chromatin interaction products in rat 
      glioma C6 cells at different phases of the cell cycle.
PG  - 110-6
AB  - The purpose of the present study was to examine the distribution pattern of 
      electron-dense acridine orange (AO) chromatin interaction products in rat glioma 
      C6 cells at different phases of the cell cycle. For synchronization in the early 
      S-phase the cells in logarithmic growth were treated with 3 micrograms/ml 
      aphidicolin, a specific inhibitor of DNA polymerase alpha and then cultured in 
      normal medium. For synchronization in the M-phase the cells cultured with 
      aphidicolin and then returned to normal medium were treated with 0.05 
      micrograms/ml colcemid. Histoautoradiographic analysis of the C6 cells using the 
      pulse chase method demonstrated approximately 16 h of cell cycle time and about 
      6.5 h of S-phase. Ultracytochemically, AO chromatin interaction products were 
      found in all phases of the cell cycle except for the mitotic phase, namely in G1, 
      S, and G2. The highest percentage of AO chromatin interaction products was 
      observed in the early S-phase and the lowest in the G2 phase. The mean number of 
      AO chromatin interaction products per nuclear area increased in the course of 
      S-phase parallel with an increase of 3H-uridine uptake during the S-phase. The 
      results show a characteristic distribution pattern of AO label specific for each 
      of the four stages of the cell cycle, however, the significance of the coincident 
      RNA synthetic activity remains to be elucidated.
FAU - Hara, H
AU  - Hara H
AD  - Department of Pathology, Kochi Medical School, Japan.
FAU - Moriki, T
AU  - Moriki T
FAU - Hiroi, M
AU  - Hiroi M
FAU - Watanabe, S
AU  - Watanabe S
FAU - Yamane, T
AU  - Yamane T
FAU - Lehmann, R
AU  - Lehmann R
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Diterpenes)
RN  - 38966-21-1 (Aphidicolin)
RN  - F30N4O6XVV (Acridine Orange)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Aphidicolin
MH  - Autoradiography
MH  - Cell Cycle/drug effects/physiology
MH  - Chromatin/*analysis
MH  - DNA, Neoplasm/*biosynthesis
MH  - Demecolcine/pharmacology
MH  - Diterpenes/pharmacology
MH  - Glioma/*ultrastructure
MH  - Microscopy, Electron
MH  - Rats
MH  - *Staining and Labeling
MH  - Tumor Cells, Cultured/drug effects/ultrastructure
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - S0344-0338(11)81018-6 [pii]
AID - 10.1016/S0344-0338(11)81018-6 [doi]
PST - ppublish
SO  - Pathol Res Pract. 1990 Feb;186(1):110-6. doi: 10.1016/S0344-0338(11)81018-6.