PMID- 16904263
OWN - NLM
STAT- MEDLINE
DCOM- 20061103
LR  - 20181201
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 406
IP  - 1-2
DP  - 2006 Oct 2
TI  - Possible involvement of BDNF release in long-lasting synapse formation induced by 
      repetitive PKA activation.
PG  - 38-42
AB  - For the analysis of the cellular mechanism underlying long-term synaptic 
      plasticity, a model system that allows long-lasting pursuit is required. 
      Previously we reported that, in hippocampal neurons under dissociated cell 
      culture conditions, repeated (but not a single) transient activation of protein 
      kinase A (PKA) led to an increase in the number of synapses that lasted >3 weeks, 
      and hence we proposed that this phenomenon should serve as an appropriate model 
      system. Here we report that repeated pulsatile application of brain-derived 
      neurotrophic factor (BDNF) leads to persistent synapse formation equivalent to 
      that after the repeated transient activation of PKA. A BDNF-scavenging substance 
      applied concomitantly with PKA activation abolished the synapse formation. The 
      release of BDNF upon PKA activation was confirmed by phosphorylation of TrkB. 
      These results indicate that the release of BDNF is involved in the putative 
      signaling cascade connecting PKA activation and synapse formation.
FAU - Taniguchi, Naoko
AU  - Taniguchi N
AD  - Department of Neuroscience, Osaka University Graduate School of Frontier 
      Biosciences, Machikaneyama-cho 1-1, Toyonaka, Osaka 560-0043, Japan.
FAU - Shinoda, Yo
AU  - Shinoda Y
FAU - Takei, Nobuyuki
AU  - Takei N
FAU - Nawa, Hiroyuki
AU  - Nawa H
FAU - Ogura, Akihiko
AU  - Ogura A
FAU - Tominaga-Yoshino, Keiko
AU  - Tominaga-Yoshino K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060809
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Synaptophysin)
RN  - 0 (Thionucleotides)
RN  - 23645-17-2 (adenosine-3',5'-cyclic phosphorothioate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Cyclic AMP/analogs & derivatives/metabolism/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/drug effects/*metabolism
MH  - Disks Large Homolog 4 Protein
MH  - Enzyme Activation/drug effects/physiology
MH  - Hippocampus/cytology/*metabolism
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Membrane Proteins/metabolism
MH  - Neural Pathways/cytology/drug effects/*metabolism
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Phosphorylation/drug effects
MH  - Presynaptic Terminals/drug effects/metabolism/ultrastructure
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/metabolism
MH  - Synapses/drug effects/*metabolism/ultrastructure
MH  - Synaptophysin/metabolism
MH  - Thionucleotides/pharmacology
EDAT- 2006/08/15 09:00
MHDA- 2006/11/04 09:00
CRDT- 2006/08/15 09:00
PHST- 2006/05/20 00:00 [received]
PHST- 2006/06/22 00:00 [revised]
PHST- 2006/06/25 00:00 [accepted]
PHST- 2006/08/15 09:00 [pubmed]
PHST- 2006/11/04 09:00 [medline]
PHST- 2006/08/15 09:00 [entrez]
AID - S0304-3940(06)00638-0 [pii]
AID - 10.1016/j.neulet.2006.06.071 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 Oct 2;406(1-2):38-42. doi: 10.1016/j.neulet.2006.06.071. Epub 
      2006 Aug 9.